TY - JOUR
T1 - Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections
AU - Higuchi, Sadanobu
AU - Suga, Moritaka
AU - Dannenberg, Arthur M.
AU - Schofield, Brian H.
PY - 1979
Y1 - 1979
N2 - Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, βgalactosidase, and cytochrome oxidase in plastic embedded and routine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 μm, were far superior to frozen Sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.
AB - Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, βgalactosidase, and cytochrome oxidase in plastic embedded and routine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 μm, were far superior to frozen Sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.
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U2 - 10.3109/10520297909110667
DO - 10.3109/10520297909110667
M3 - Article
C2 - 90411
AN - SCOPUS:84907129197
SN - 1052-0295
VL - 54
SP - 5
EP - 12
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 1
ER -