Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections

Sadanobu Higuchi, Moritaka Suga, Arthur M. Dannenberg, Brian H. Schofield

Research output: Contribution to journalArticle

Abstract

Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, βgalactosidase, and cytochrome oxidase in plastic embedded and routine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 μm, were far superior to frozen Sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.

Original languageEnglish (US)
Pages (from-to)5-12
Number of pages8
JournalBiotechnic and Histochemistry
Volume54
Issue number1
DOIs
StatePublished - 1979

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Frozen Sections
Paraffin
Plastics
Galactosidases
Methacrylates
Electron Transport Complex IV
Esterases
Enzymes
Acid Phosphatase
Staining and Labeling
Water

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Cite this

Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections. / Higuchi, Sadanobu; Suga, Moritaka; Dannenberg, Arthur M.; Schofield, Brian H.

In: Biotechnic and Histochemistry, Vol. 54, No. 1, 1979, p. 5-12.

Research output: Contribution to journalArticle

Higuchi, Sadanobu ; Suga, Moritaka ; Dannenberg, Arthur M. ; Schofield, Brian H. / Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections. In: Biotechnic and Histochemistry. 1979 ; Vol. 54, No. 1. pp. 5-12.
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