Histamine stimulation of the nasal mucosa does not induce prostaglandin or leukotriene generation or induce methacholine hyperresponsiveness

Abraham M. Majchel, David Proud, Walter C. Hubbard, Robert M. Naclerio

Research output: Contribution to journalArticle

Abstract

To further define the role of histamine in the nasal mucosa, we studied the possible effect of histamine provocation on the generation of prostanoids and leukotrienes, and on the induction of hyperresponsiveness to methacholine. In separate experiments, we performed nasal challenges with histamine and measured by gas chromatography negative ion mass spectrometry and by radioimmunoassay after high-performance liquid chromatography the levels of prostanoids and leukotrienes, respectively, in recovered nasal lavages 10 min after challenge. Hyperresponsiveness to methacholine was tested in both nostrils 24 h after unilateral provocation with histamine. Our data suggest that histamine induced an immediate symptomatic response, but neither led to the generation of prostaglandins or leukotrienes nor induced hyperresponsiveness to methacholine. These results differ from those achieved after antigen stimulation and emphasize the importance of mediators in addition to histamine in the allergic reaction.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume95
Issue number2-3
DOIs
StatePublished - 1991

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Methacholine Chloride
Nasal Mucosa
Leukotrienes
Histamine
Prostaglandins
Nasal Lavage
Nose
Gas Chromatography
Radioimmunoassay
Mass Spectrometry
Hypersensitivity
High Pressure Liquid Chromatography
Ions
Antigens

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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Histamine stimulation of the nasal mucosa does not induce prostaglandin or leukotriene generation or induce methacholine hyperresponsiveness. / Majchel, Abraham M.; Proud, David; Hubbard, Walter C.; Naclerio, Robert M.

In: International Archives of Allergy and Immunology, Vol. 95, No. 2-3, 1991, p. 149-155.

Research output: Contribution to journalArticle

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