Hindered rotational diffusion and rotational jumps of single molecules

Taekjip Ha, J. Glass, Th Enderle, D. S. Chemla, S. Weiss

Research output: Contribution to journalArticle

Abstract

We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.

Original languageEnglish (US)
Pages (from-to)2093-2096
Number of pages4
JournalPhysical Review Letters
Volume80
Issue number10
StatePublished - 1998
Externally publishedYes

Fingerprint

molecular interactions
polarization
molecules
fluorescence
anisotropy
sensitivity
liquids

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Ha, T., Glass, J., Enderle, T., Chemla, D. S., & Weiss, S. (1998). Hindered rotational diffusion and rotational jumps of single molecules. Physical Review Letters, 80(10), 2093-2096.

Hindered rotational diffusion and rotational jumps of single molecules. / Ha, Taekjip; Glass, J.; Enderle, Th; Chemla, D. S.; Weiss, S.

In: Physical Review Letters, Vol. 80, No. 10, 1998, p. 2093-2096.

Research output: Contribution to journalArticle

Ha, T, Glass, J, Enderle, T, Chemla, DS & Weiss, S 1998, 'Hindered rotational diffusion and rotational jumps of single molecules', Physical Review Letters, vol. 80, no. 10, pp. 2093-2096.
Ha T, Glass J, Enderle T, Chemla DS, Weiss S. Hindered rotational diffusion and rotational jumps of single molecules. Physical Review Letters. 1998;80(10):2093-2096.
Ha, Taekjip ; Glass, J. ; Enderle, Th ; Chemla, D. S. ; Weiss, S. / Hindered rotational diffusion and rotational jumps of single molecules. In: Physical Review Letters. 1998 ; Vol. 80, No. 10. pp. 2093-2096.
@article{edd716a8877a452c88d77e9c7cffdbfb,
title = "Hindered rotational diffusion and rotational jumps of single molecules",
abstract = "We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.",
author = "Taekjip Ha and J. Glass and Th Enderle and Chemla, {D. S.} and S. Weiss",
year = "1998",
language = "English (US)",
volume = "80",
pages = "2093--2096",
journal = "Physical Review Letters",
issn = "0031-9007",
publisher = "American Physical Society",
number = "10",

}

TY - JOUR

T1 - Hindered rotational diffusion and rotational jumps of single molecules

AU - Ha, Taekjip

AU - Glass, J.

AU - Enderle, Th

AU - Chemla, D. S.

AU - Weiss, S.

PY - 1998

Y1 - 1998

N2 - We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.

AB - We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.

UR - http://www.scopus.com/inward/record.url?scp=0000381357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000381357&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0000381357

VL - 80

SP - 2093

EP - 2096

JO - Physical Review Letters

JF - Physical Review Letters

SN - 0031-9007

IS - 10

ER -