Hindered rotational diffusion and rotational jumps of single molecules

T. Ha, J. Glass, T. H. Enderle, D. S. Chemla, S. Weiss

Research output: Contribution to journalArticle

Abstract

We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.

Original languageEnglish (US)
Pages (from-to)2093-2096
Number of pages4
JournalPhysical Review Letters
Volume80
Issue number10
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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