Hilar interneuron vulnerability distinguishes aged rats with memory impairment

Amy M. Spiegel, Ming Teng Koh, Nicholas M. Vogt, Peter R. Rapp, Michela Gallagher

Research output: Contribution to journalArticle

Abstract

Hippocampal interneuron populations are reportedly vulnerable to normal aging. The relationship between interneuron network integrity and age-related memory impairment, however, has not been tested directly. That question was addressed in the present study using a well-characterized model in which outbred, aged, male Long-Evans rats exhibit a spectrum of individual differences in hippocampal-dependent memory. Selected interneuron populations in the hippocampus were visualized for stereological quantification with a panel of immunocytochemical markers, including glutamic acid decarboxylase-67 (GAD67), somatostatin, and neuropeptide Y. The overall pattern of results was that, although the numbers of GAD67- and somatostatin-positive interneurons declined with age across multiple fields of the hippocampus, alterations specifically related to the cognitive outcome of aging were observed exclusively in the hilus of the dentate gyrus. Because the total number of NeuN-immunoreactive hilar neurons was unaffected, the decline observed with other markers likely reflects a loss of target protein rather than neuron death. In support of that interpretation, treatment with the atypical antiepileptic levetiracetam at a low dose shown previously to improve behavioral performance fully restored hilar SOM expression in aged, memory-impaired rats. Age-related decreases in GAD67- and somatostatin-immunoreactive neuron number beyond the hilus were regionally selective and spared the CA1 field of the hippocampus entirely. Together these findings confirm the vulnerability of hippocampal interneurons to normal aging and highlight that the integrity of a specific subpopulation in the hilus is coupled with age-related memory impairment.

Original languageEnglish (US)
Pages (from-to)3508-3523
Number of pages16
JournalJournal of Comparative Neurology
Volume521
Issue number15
DOIs
StatePublished - Oct 15 2013

Fingerprint

Interneurons
Glutamate Decarboxylase
Somatostatin
etiracetam
Neurons
Hippocampus
Hippocampal CA1 Region
Long Evans Rats
Neuropeptide Y
Dentate Gyrus
Vulnerable Populations
Individuality
Anticonvulsants
Population
Proteins

Keywords

  • Hippocampus
  • Interneuron
  • Levetiracetam
  • Neuropeptide Y
  • Somatostatin
  • Stereology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hilar interneuron vulnerability distinguishes aged rats with memory impairment. / Spiegel, Amy M.; Koh, Ming Teng; Vogt, Nicholas M.; Rapp, Peter R.; Gallagher, Michela.

In: Journal of Comparative Neurology, Vol. 521, No. 15, 15.10.2013, p. 3508-3523.

Research output: Contribution to journalArticle

Spiegel, Amy M. ; Koh, Ming Teng ; Vogt, Nicholas M. ; Rapp, Peter R. ; Gallagher, Michela. / Hilar interneuron vulnerability distinguishes aged rats with memory impairment. In: Journal of Comparative Neurology. 2013 ; Vol. 521, No. 15. pp. 3508-3523.
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