Highly sensitive differential pulse amperometric detection of second generation anti-tumor platinum compounds in HPLC effluents

W. J F van der Vijgh, H. B J van der Lee, G. J. Postma, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

A highly sensitive on-line amperometric detection of platinum compounds in HPLC effluents was possible with the use of a polarographic detector. For TNO-1 (=cis-1,1-di(aminomethyl)-cyclohexane Pt(II)chloride) a linear dynamic range of at least three decades could be obtained (0.034-108μg TNO-1/ml) with a detection limit of three times the noise signal at 0.7 ng TNO-1 (20ng Pt/ml). The sensitivity was 0.1 nA/ng TNO-1 (0.2nA/ng Pt). The within-day presicion was 1.1% at a concentration of 10.8μg TNO-1/ml (n=10). With this system the compatibility was measured of TNO-6 with infusion fluids. In 0.15 M NaCl, TNO-6 (=cis-1,1-di(aminomethyl)-cyclohexanePt(II)sulphate) was converted into TNO-1 within 40 minutes. Molecular changes of TNO-6 were also observed in a 5% aqueous glucose solution.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalChromatographia
Volume17
Issue number6
DOIs
StatePublished - Jun 1983
Externally publishedYes

Keywords

  • anti-tumor platinum compounds
  • differential pulse amperometry
  • HPLC retention
  • sensitive on-line detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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