A highly sensitive on-line amperometric detection of platinum compounds in HPLC effluents was possible with the use of a polarographic detector. For TNO-1 (=cis-1,1-di(aminomethyl)-cyclohexane Pt(II)chloride) a linear dynamic range of at least three decades could be obtained (0.034-108μg TNO-1/ml) with a detection limit of three times the noise signal at 0.7 ng TNO-1 (20ng Pt/ml). The sensitivity was 0.1 nA/ng TNO-1 (0.2nA/ng Pt). The within-day presicion was 1.1% at a concentration of 10.8μg TNO-1/ml (n=10). With this system the compatibility was measured of TNO-6 with infusion fluids. In 0.15 M NaCl, TNO-6 (=cis-1,1-di(aminomethyl)-cyclohexanePt(II)sulphate) was converted into TNO-1 within 40 minutes. Molecular changes of TNO-6 were also observed in a 5% aqueous glucose solution.
- anti-tumor platinum compounds
- differential pulse amperometry
- HPLC retention
- sensitive on-line detection
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry