TY - JOUR
T1 - Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid
AU - Cuaranta-Monroy, Ixchelt
AU - Simandi, Zoltan
AU - Kolostyak, Zsuzsanna
AU - Doan-Xuan, Quang Minh
AU - Poliska, Szilard
AU - Horvath, Attila
AU - Nagy, Gergely
AU - Bacso, Zsolt
AU - Nagy, Laszlo
N1 - Funding Information:
The authors would like to acknowledge the members of the Nagy laboratory, Dr. Gerardo Alvarado and Dr. Istvan Szatmari for discussions and comments on the manuscript. L.N. is supported by a grant from the Hungarian Scientific Research Fund ( OTKA K100196 ). L.N. and I.C.-M. are recipients of the following grant TÁMOP-4.2.2.A-11/1/KONV-2012-0023 VÉD-ELEM implemented through the New Hungary Development Plan co-financed by the European Social Fund and the European Regional Development Fund . Z.S. and G.N. are recipients of TÁMOP 4.2.4.A/2-11-1-2012-0001/A2-JÁDJ-13 . Microarray studies and Next Generation Sequencing were carried out at the Center for Clinical Genomics and Personalized Medicine at the University of Debrecen, Medical and Health Science Center.
PY - 2014/7
Y1 - 2014/7
N2 - Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells are in a monolayer, allowing a broad range of genome-wide studies in early and late stages of adipocyte differentiation to be performed.
AB - Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells are in a monolayer, allowing a broad range of genome-wide studies in early and late stages of adipocyte differentiation to be performed.
UR - http://www.scopus.com/inward/record.url?scp=84901234710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901234710&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2014.04.015
DO - 10.1016/j.scr.2014.04.015
M3 - Article
C2 - 24858493
AN - SCOPUS:84901234710
SN - 1873-5061
VL - 13
SP - 88
EP - 97
JO - Stem Cell Research
JF - Stem Cell Research
IS - 1
ER -