Highly dynamic transcriptional signature of distinct macrophage subsets during sterile inflammation, resolution, and tissue repair

Tamas Varga, Remi Mounier, Attila Horvath, Sylvain Cuvellier, Florent Dumont, Szilard Poliska, Hamida Ardjoune, Gaetan Juban, Laszlo Nagy, Benedicte Chazaud

Research output: Contribution to journalArticle

Abstract

Macrophage gene expression determines phagocyte responses and effector functions. Macrophage plasticity has been mainly addressed in in vitro models that do not account for the environmental complexity observed in vivo. In this study, we show that microarray gene expression profiling revealed a highly dynamic landscape of transcriptomic changes of Ly6CposCX3CR1lo and Ly6CnegCX3CR1hi macrophage populations during skeletal muscle regeneration after a sterile damage. Systematic gene expression analysis revealed that the time elapsed, much more than Ly6C status, was correlated with the largest differential gene expression, indicating that the time course of inflammation was the predominant driving force of macrophage gene expression. Moreover, Ly6Cpos/Ly6Cneg subsets could not have been aligned to canonical M1/M2 profiles. Instead, a combination of analyses suggested the existence of four main features of muscle-derived macrophages specifying important steps of regeneration: 1) infiltrating Ly6Cpos macrophages expressed acute-phase proteins and exhibited an inflammatory profile independent of IFN-g, making them damage-associated macrophages; 2) metabolic changes of macrophages, characterized by a decreased glycolysis and an increased tricarboxylic acid cycle/oxidative pathway, preceded the switch to and sustained their anti-inflammatory profile; 3) Ly6Cneg macrophages, originating from skewed Ly6Cpos cells, actively proliferated; and 4) later on, restorative Ly6Cneg macrophages were characterized by a novel profile, indicative of secretion of molecules involved in intercellular communications, notably matrix-related molecules. These results show the highly dynamic nature of the macrophage response at the molecular level after an acute tissue injury and subsequent repair, and associate a specific signature of macrophages to predictive specialized functions of macrophages at each step of tissue injury/repair.

Original languageEnglish (US)
Pages (from-to)4771-4782
Number of pages12
JournalJournal of Immunology
Volume196
Issue number11
DOIs
StatePublished - Jun 1 2016
Externally publishedYes

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Macrophages
Inflammation
Gene Expression
Regeneration
Citric Acid Cycle
Acute-Phase Proteins
Wounds and Injuries
Gene Expression Profiling
Glycolysis
Phagocytes
Skeletal Muscle
Anti-Inflammatory Agents
Muscles

ASJC Scopus subject areas

  • Immunology

Cite this

Highly dynamic transcriptional signature of distinct macrophage subsets during sterile inflammation, resolution, and tissue repair. / Varga, Tamas; Mounier, Remi; Horvath, Attila; Cuvellier, Sylvain; Dumont, Florent; Poliska, Szilard; Ardjoune, Hamida; Juban, Gaetan; Nagy, Laszlo; Chazaud, Benedicte.

In: Journal of Immunology, Vol. 196, No. 11, 01.06.2016, p. 4771-4782.

Research output: Contribution to journalArticle

Varga, T, Mounier, R, Horvath, A, Cuvellier, S, Dumont, F, Poliska, S, Ardjoune, H, Juban, G, Nagy, L & Chazaud, B 2016, 'Highly dynamic transcriptional signature of distinct macrophage subsets during sterile inflammation, resolution, and tissue repair', Journal of Immunology, vol. 196, no. 11, pp. 4771-4782. https://doi.org/10.4049/jimmunol.1502490
Varga, Tamas ; Mounier, Remi ; Horvath, Attila ; Cuvellier, Sylvain ; Dumont, Florent ; Poliska, Szilard ; Ardjoune, Hamida ; Juban, Gaetan ; Nagy, Laszlo ; Chazaud, Benedicte. / Highly dynamic transcriptional signature of distinct macrophage subsets during sterile inflammation, resolution, and tissue repair. In: Journal of Immunology. 2016 ; Vol. 196, No. 11. pp. 4771-4782.
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