Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Alice M. Delvolve; Benoit Colsch; Amina S. Woods

doi:10.1039/c1ay05107e

Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Alice M. Delvolve, Benoit Colsch, Amina S. Woods

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Cell membranes are made up of a mixture of glycerolipids, sphingolipids, gangliosides and cholesterol. Lipids play important roles in a cell's life. However many of their functions have still to be discovered. In the present work, we describe an efficient, easy and rapid methodology to accurately localize phosphatidylcholines and sphingomyelins from a single coronal rat brain section in the cerebrum area. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was used to profile and image lipids. The best resolved structure was 25-50 μm in the hippocampus.

Original language	English (US)
Pages (from-to)	1729-1736
Number of pages	8
Journal	Analytical Methods
Volume	3
Issue number	8
DOIs	https://doi.org/10.1039/c1ay05107e
State	Published - Aug 2011
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
General Engineering
General Chemical Engineering

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10.1039/c1ay05107e

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AB - Cell membranes are made up of a mixture of glycerolipids, sphingolipids, gangliosides and cholesterol. Lipids play important roles in a cell's life. However many of their functions have still to be discovered. In the present work, we describe an efficient, easy and rapid methodology to accurately localize phosphatidylcholines and sphingomyelins from a single coronal rat brain section in the cerebrum area. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was used to profile and image lipids. The best resolved structure was 25-50 μm in the hippocampus.

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Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Abstract

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