Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Alice M. Delvolve, Benoit Colsch, Amina S. Woods

Research output: Contribution to journalArticlepeer-review


Cell membranes are made up of a mixture of glycerolipids, sphingolipids, gangliosides and cholesterol. Lipids play important roles in a cell's life. However many of their functions have still to be discovered. In the present work, we describe an efficient, easy and rapid methodology to accurately localize phosphatidylcholines and sphingomyelins from a single coronal rat brain section in the cerebrum area. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was used to profile and image lipids. The best resolved structure was 25-50 μm in the hippocampus.

Original languageEnglish (US)
Pages (from-to)1729-1736
Number of pages8
JournalAnalytical Methods
Issue number8
StatePublished - Aug 2011
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)


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