High throughput screening technologies for ion channels

Hai Bo Yu, Min Li, Wei Ping Wang, Xiao Liang Wang

Research output: Contribution to journalReview article

Abstract

Ion channels are involved in a variety of fundamental physiological processes, and their malfunction causes numerous human diseases. Therefore, ion channels represent a class of attractive drug targets and a class of important off-targets for in vitro pharmacological profiling. In the past decades, the rapid progress in developing functional assays and instrumentation has enabled high throughput screening (HTS) campaigns on an expanding list of channel types. Chronologically, HTS methods for ion channels include the ligand binding assay, flux-based assay, fluorescence-based assay, and automated electrophysiological assay. In this review we summarize the current HTS technologies for different ion channel classes and their applications.

Original languageEnglish (US)
Pages (from-to)34-43
Number of pages10
JournalActa Pharmacologica Sinica
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Ion Channels
Technology
High-Throughput Screening Assays
Physiological Phenomena
Fluorescence
Pharmacology
Ligands
Pharmaceutical Preparations

Keywords

  • automated electrophysiological assay
  • drug discovery
  • fluorescence-based assay
  • flux-based assay
  • high throughput screening
  • ion channels
  • ligand binding assay

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

High throughput screening technologies for ion channels. / Yu, Hai Bo; Li, Min; Wang, Wei Ping; Wang, Xiao Liang.

In: Acta Pharmacologica Sinica, Vol. 37, No. 1, 01.01.2016, p. 34-43.

Research output: Contribution to journalReview article

Yu, Hai Bo ; Li, Min ; Wang, Wei Ping ; Wang, Xiao Liang. / High throughput screening technologies for ion channels. In: Acta Pharmacologica Sinica. 2016 ; Vol. 37, No. 1. pp. 34-43.
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