High-throughput RNA in situ hybridization in mouse retina

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.

Original languageEnglish (US)
Title of host publicationRetinal Degeneration
Subtitle of host publicationMethods and Protocols
EditorsBernhard H.F. Weber, Thomas Langmann
Pages215-226
Number of pages12
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume935
ISSN (Print)1064-3745

Keywords

  • Cellular resolution
  • Chromogenic
  • Development
  • Digoxigenin
  • Gene expression
  • Hybridization
  • Photoreceptor
  • RNA
  • Retina
  • Riboprobe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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