High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array

Yi Zhang, Vasudev Bailey, Christopher M. Puleo, Hariharan Easwaran, Elizabeth Griffiths, James G. Herman, Stephen B Baylin, Tza Huei Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages806-808
Number of pages3
DOIs
StatePublished - 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Fingerprint

Methylation
Polymerase chain reaction
Throughput
Microfluidics
Tumors
Assays
Fluids
Oils
DNA Methylation

Keywords

  • DNA methylation
  • Droplet-in-oil
  • PCR

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Zhang, Y., Bailey, V., Puleo, C. M., Easwaran, H., Griffiths, E., Herman, J. G., ... Wang, T. H. (2009). High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 806-808). [5285699] https://doi.org/10.1109/SENSOR.2009.5285699

High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. / Zhang, Yi; Bailey, Vasudev; Puleo, Christopher M.; Easwaran, Hariharan; Griffiths, Elizabeth; Herman, James G.; Baylin, Stephen B; Wang, Tza Huei.

TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 806-808 5285699.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, Y, Bailey, V, Puleo, CM, Easwaran, H, Griffiths, E, Herman, JG, Baylin, SB & Wang, TH 2009, High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285699, pp. 806-808, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 6/21/09. https://doi.org/10.1109/SENSOR.2009.5285699
Zhang Y, Bailey V, Puleo CM, Easwaran H, Griffiths E, Herman JG et al. High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 806-808. 5285699 https://doi.org/10.1109/SENSOR.2009.5285699
Zhang, Yi ; Bailey, Vasudev ; Puleo, Christopher M. ; Easwaran, Hariharan ; Griffiths, Elizabeth ; Herman, James G. ; Baylin, Stephen B ; Wang, Tza Huei. / High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 806-808
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