High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array

Yi Zhang, Vasudev Bailey, Christopher M. Puleo, Hariharan Easwaran, Elizabeth Griffiths, James G. Herman, Stephen B. Baylin, Tza Huei Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15 and TMS1 using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages806-808
Number of pages3
DOIs
StatePublished - Dec 11 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Keywords

  • DNA methylation
  • Droplet-in-oil
  • PCR

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array'. Together they form a unique fingerprint.

  • Cite this

    Zhang, Y., Bailey, V., Puleo, C. M., Easwaran, H., Griffiths, E., Herman, J. G., Baylin, S. B., & Wang, T. H. (2009). High throughput DNA methylation analysis on a droplet-in-oil polymerase chain reaction array. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 806-808). [5285699] (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285699