@inbook{410c2d01fcb24194965429f086a00834,
title = "High-Throughput Analysis of Glycoproteins from Plasma",
abstract = "The glycoproteome is a major subproteome of plasma. To identify low-abundance plasma glycoproteins and specific glycoforms as disease biomarkers, high-throughput and reproducible methods for glycoproteomic analysis are needed. A glycoproteomic method, utilising solid-phase extraction of glycopeptides (SPEG) based on chemical immobilization of glycopeptides has been developed. The step-by-step protocol for this high-throughput SPEG method using multichannel liquid handling system is described. The protocol provides efficient enrichment for glycoproteins for proteomic identification using mass spectrometry.",
keywords = "Glycoprotein, Mass spectrometry, Solid-phase extraction of glycopeptides",
author = "Yan Li and Hui Zhang",
note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-068-3_7",
language = "English (US)",
isbn = "9781617790676",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "125--133",
booktitle = "Serum/Plasma Proteomics",
}