High-throughput analysis of glycoproteins from plasma

Yan Li, Hui Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The glycoproteome is a major subproteome of plasma. To identify low-abundance plasma glycoproteins and specific glycoforms as disease biomarkers, high-throughput and reproducible methods for glycoproteomic analysis are needed. A glycoproteomic method, utilising solid-phase extraction of glycopeptides (SPEG) based on chemical immobilization of glycopeptides has been developed. The step-by-step protocol for this high-throughput SPEG method using multichannel liquid handling system is described. The protocol provides efficient enrichment for glycoproteins for proteomic identification using mass spectrometry.

Original languageEnglish (US)
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsRichard Simpson, David Greening
Pages125-133
Number of pages9
DOIs
StatePublished - Dec 1 2011

Publication series

NameMethods in Molecular Biology
Volume728
ISSN (Print)1064-3745

Keywords

  • Glycoprotein
  • Mass spectrometry
  • Solid-phase extraction of glycopeptides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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