High-speed fiber-optic scanning nonlinear endomicroscopy for imaging neuron dynamics in vivo

Hyeon Cheol Park, Honghua Guan, Ang Li, Yuanlei Yue, Ming Jun Li, Hui Lu, Xingde Li

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Fiber-optic-based two-photon fluorescence endomicroscopy is emerging as an enabling technology for in vivo histological imaging of internal organs and functional neuronal imaging on freely-behaving animals. However, high-speed imaging remains challenging due to the expense of miniaturization and lack of suited fast beam scanners. For many applications, a higher imaging speed is highly desired, especially for monitoring functional dynamics such as transient dendritic responses in neuroscience. This Letter reports the development of a fast fiber-optic scanning endomicroscope with an imaging speed higher than 26 frames/s. In vivo neural dynamics imaging with the high-speed endomicroscope was performed on a freely-behaving mouse over the primary motor cortex that expressed GCaMP6m. The results demonstrate its capability of real-time monitoring of transient neuronal dynamics with very fine temporal resolution.

Original languageEnglish (US)
Pages (from-to)3605-3608
Number of pages4
JournalOptics Letters
Issue number13
StatePublished - Jul 1 2020

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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