High-sensitivity cerebral perfusion mapping in mice by kbGRASE-FAIR at 9.4 T

Bingwen Zheng, Philip Teck Hock Lee, Xavier Golay

Research output: Contribution to journalArticle

Abstract

The combination of flow-sensitive alternating inversion recovery (FAIR) and single-shot k-space-banded gradient- and spin-echo (kbGRASE) is proposed here to measure perfusion in the mouse brain with high sensitivity and stability. Signal-to-noise ratio (SNR) analysis showed that kbGRASE-FAIR boosts image and temporal SNRs by 2.01±0.08 and 2.50±0.07 times, respectively, when compared with standard single-shot echo planar imaging (EPI)-FAIR implemented in our experimental systems, although the practically achievable spatial resolution was slightly reduced. The effects of varying physiological parameters on the precision and reproducibility of cerebral blood flow (CBF) measurements were studied following changes in anesthesia regime, capnia and body temperature. The functional MRI time courses with kbGRASE-FAIR showed a more stable response to 5% CO2 than did those with EPI-FAIR. The results establish kbGRASE-FAIR as a practical and robust protocol for quantitative CBF measurements in mice at 9.4T. The aim of this study was to develop a practical perfusion measurement with high sensitivity and stability in mouse brain at high field strength by the combination of flow-sensitive alternating inversion recovery (FAIR) and single-shot k-space-banded gradient- and spin-echo (kbGRASE). To estimate the influence of physiological parameters on the precision and reproducibility of cerebral blood flow (CBF) measurements, changes in anesthesia regime, capnia and body temperature were performed.

Original languageEnglish (US)
Pages (from-to)1061-1070
Number of pages10
JournalNMR in biomedicine
Volume23
Issue number9
DOIs
StatePublished - Nov 1 2010

Keywords

  • Arterial spin labeling
  • Cerebral blood flow
  • KbGRASE
  • MRI
  • Mouse brain

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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