High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area

Weimin Jiang, Lingyun Shao, Ying Zhang, Shu Zhang, Chengyan Meng, Yunya Xu, Lingli Huang, Yun Wang, Ying Wang, Xinhua Weng, Wenhong Zhang

Research output: Contribution to journalArticle

Abstract

Background: An accurate test for Mycobacterium tuberculosis infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-γ release assay in detecting active and latent tuberculosis in HIV-infected population in bacillus Calmette-Guerin (BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-γ release assay, T-SPOT.TB, was used to evaluate the M. tuberculosis ESAT-6 and CFP-10 specific IFN-γ response. Tuberculin skin test (TST) was performed for all recruited subjects. Results: The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST 85% in patients with TB treatment for less than 1 month and CD4+ T cells ≥200/μl, while for patients treated for more than 3 months and CD4+ T cells

Original languageEnglish (US)
Article number31
JournalBMC Immunology
Volume10
DOIs
StatePublished - May 28 2009

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Mycobacterium Infections
Mycobacterium bovis
Mycobacterium tuberculosis
HIV
Tuberculin Test
Tuberculosis
Skin Tests
Latent Tuberculosis
T-Lymphocytes
Enzymes
Population

ASJC Scopus subject areas

  • Immunology

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High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area. / Jiang, Weimin; Shao, Lingyun; Zhang, Ying; Zhang, Shu; Meng, Chengyan; Xu, Yunya; Huang, Lingli; Wang, Yun; Wang, Ying; Weng, Xinhua; Zhang, Wenhong.

In: BMC Immunology, Vol. 10, 31, 28.05.2009.

Research output: Contribution to journalArticle

Jiang, Weimin ; Shao, Lingyun ; Zhang, Ying ; Zhang, Shu ; Meng, Chengyan ; Xu, Yunya ; Huang, Lingli ; Wang, Yun ; Wang, Ying ; Weng, Xinhua ; Zhang, Wenhong. / High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area. In: BMC Immunology. 2009 ; Vol. 10.
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abstract = "Background: An accurate test for Mycobacterium tuberculosis infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-γ release assay in detecting active and latent tuberculosis in HIV-infected population in bacillus Calmette-Guerin (BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-γ release assay, T-SPOT.TB, was used to evaluate the M. tuberculosis ESAT-6 and CFP-10 specific IFN-γ response. Tuberculin skin test (TST) was performed for all recruited subjects. Results: The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST 85{\%} in patients with TB treatment for less than 1 month and CD4+ T cells ≥200/μl, while for patients treated for more than 3 months and CD4+ T cells",
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