High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA

Duane B. Lakings, T. Phillip Waalkes, John E. Mrochek

Research output: Contribution to journalArticlepeer-review

Abstract

Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalJournal of Chromatography A
Volume116
Issue number1
DOIs
StatePublished - Jan 7 1976

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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