Abstract
Methylated and major purine and pyridine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the h ydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated samples of tRNA were hydrolyzed to the free bases with a triluoroacetic acid-formic acid mixture at 200°. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.
Original language | English (US) |
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Pages (from-to) | 83-92 |
Number of pages | 10 |
Journal | Journal of Chromatography A |
Volume | 116 |
Issue number | 1 |
DOIs | |
State | Published - Jan 7 1976 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry