High resolution electron paramagnetic resonance imaging of biological samples with a single line paramagnetic label

Periannan Kuppusamy, Penghai Wang, Michael Chzhan, Jay L. Zweier

Research output: Contribution to journalArticlepeer-review

Abstract

The application of electron paramagnetic resonance imaging (EPRI) to obtain information from biological samples has been limited by the lack of ideal single line radical labels. The commonly used nitroxides exhibit multiple lines causing either hyperfine-based limitations in the maximum obtainable image resolution or hyperfine-based artifacts in the reconstructed image. The use of a novel single-line triarylmethyl paramagnetic label that enables marked enhancement in image quality and resolution is reported. This label exhibits a single line EPR spectrum that is sharp (linewidth ~60 mG in the absence of oxygen) and relatively stable in tissues. The potential of this label in enabling high resolution EPR imaging of biological samples was demonstrated in a series of phantoms and isolated biological organs such as the rat kidney. The images demonstrate that resolutions better than 100 μm could be obtained at L-band on samples of up to 20 mm in size.

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalMagnetic Resonance in Medicine
Volume37
Issue number4
DOIs
StatePublished - Apr 1997

Keywords

  • EPR imaging
  • free radical
  • image resolution
  • rat kidney

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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