High resolution deuterium NMR studies of bacterial metabolism

J. B. Aguayo, M. P. Gamcsik, J. D. Dick

Research output: Contribution to journalArticle

Abstract

High resolution deuterium NMR spectra were obtained from suspensions of five bacterial strains: Escherichia coli, Clostridium perfringens, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus. Deuterium-labeled D-glucose at C-1, C-2, and C-6 was used to monitor dynamically anaerobic metabolism. The flux of glucose through the various bacterial metabolic pathways could be determined by following the disappearance of glucose and the appearance of the major end products in the 2H NMR spectrum. The presence of both labeled and unlabeled metabolites could be detected using 1H NMR spectroscopy since the proton resonances in the labeled species are shifted upfield due to an isotopic chemical shift effect. The 1H-1H scalar coupling observed in both the 2H and 1H NMR spectra was used to assign definitively the resonances of labeled species. An increase in the intensity of natural abundance deuterium signal of water can be used to monitor pathways in which a deuteron is lost from the labeled metabolite. The steps in which label loss can occur are outlined, and the influence these processes have on the ability of 2H NMR spectroscopy to monitor metabolism are assessed.

Original languageEnglish (US)
Pages (from-to)19552-19557
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number36
StatePublished - 1988

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Deuterium
Metabolism
Nuclear magnetic resonance
Metabolites
Glucose
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Anaerobiosis
Proteus mirabilis
Clostridium
Clostridium perfringens
Klebsiella pneumoniae
Chemical shift
Metabolic Networks and Pathways
Escherichia coli
Staphylococcus aureus
Protons
Labels
Suspensions
Fluxes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aguayo, J. B., Gamcsik, M. P., & Dick, J. D. (1988). High resolution deuterium NMR studies of bacterial metabolism. Journal of Biological Chemistry, 263(36), 19552-19557.

High resolution deuterium NMR studies of bacterial metabolism. / Aguayo, J. B.; Gamcsik, M. P.; Dick, J. D.

In: Journal of Biological Chemistry, Vol. 263, No. 36, 1988, p. 19552-19557.

Research output: Contribution to journalArticle

Aguayo, JB, Gamcsik, MP & Dick, JD 1988, 'High resolution deuterium NMR studies of bacterial metabolism', Journal of Biological Chemistry, vol. 263, no. 36, pp. 19552-19557.
Aguayo, J. B. ; Gamcsik, M. P. ; Dick, J. D. / High resolution deuterium NMR studies of bacterial metabolism. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 36. pp. 19552-19557.
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