High-Resolution Analysis of Antibodies to Post-Translational Modifications Using Peptide Nanosensor Microarrays

Jung Rok Lee, D. James Haddon, Nidhi Gupta, Jordan V. Price, Grace M. Credo, Vivian K. Diep, Kyunglok Kim, Drew A. Hall, Emily C. Baechler, Michelle Petri, Madoo Varma, Paul J. Utz, Shan X. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Autoantibodies are a hallmark of autoimmune diseases such as lupus and have the potential to be used as biomarkers for diverse diseases, including immunodeficiency, infectious disease, and cancer. More precise detection of antibodies to specific targets is needed to improve diagnosis of such diseases. Here, we report the development of reusable peptide microarrays, based on giant magnetoresistive (GMR) nanosensors optimized for sensitively detecting magnetic nanoparticle labels, for the detection of antibodies with a resolution of a single post-translationally modified amino acid. We have also developed a chemical regeneration scheme to perform multiplex assays with a high level of reproducibility, resulting in greatly reduced experimental costs. In addition, we show that peptides synthesized directly on the nanosensors are approximately two times more sensitive than directly spotted peptides. Reusable peptide nanosensor microarrays enable precise detection of autoantibodies with high resolution and sensitivity and show promise for investigating antibody-mediated immune responses to autoantigens, vaccines, and pathogen-derived antigens as well as other fundamental peptide-protein interactions.

Original languageEnglish (US)
Pages (from-to)10652-10660
Number of pages9
JournalACS Nano
Volume10
Issue number12
DOIs
StatePublished - Dec 27 2016

Keywords

  • autoantibody
  • giant magnetoresistance
  • lupus
  • nanosensors
  • peptide microarray
  • regeneration

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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