High precision profiling of glycopeptides using HPLC-CHIP/MS TOF

Kevin Killeen, Hongfeng Yin, Reid Brennen, Karen Seaward, Rudi Grimm, Xiao Jun Li, Hui Zhang, Ruedi Aebersold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Protein glycosylation is critically important to biological function and disease state. An effective method for the selective isolation of N-linked glycopeptides has been developed, based on the conjugation of glycoproteins to a solid support using hydrazine chemistry [1]. Due to extreme sample complexity, separation methods such as nano-LC/MS has been used as an analytical tool for human serum samples but we illustrate here that a microfluidic device can achieve superior performance in both sensitivity and reproducibility for profiling complex samples such as glycosylated proteins.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages274-276
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

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ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Killeen, K., Yin, H., Brennen, R., Seaward, K., Grimm, R., Li, X. J., Zhang, H., & Aebersold, R. (2005). High precision profiling of glycopeptides using HPLC-CHIP/MS TOF. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 274-276). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.