High Precision DNA Modification Analysis of HCG9 in Major Psychosis

Mrinal Pal, Sasha Ebrahimi, Gabriel Oh, Tarang Khare, Aiping Zhang, Zachary A. Kaminsky, Sun Chong Wang, Arturas Petronis

Research output: Contribution to journalArticle

Abstract

New epigenetic technologies may uncover etiopathogenic mechanisms of major psychosis. In this study, we applied padlock probe-based ultra-deep bisulfite sequencing for fine mapping of modified cytosines of the HLA complex group 9 (nonprotein coding) gene in the postmortem brains of individuals affected with schizophrenia or bipolar disorder and unaffected controls. Significant differences between patients and controls were detected in both CpG and CpH modifications. In addition, we identified epigenetic age effects, DNA modification differences between sense and anti-sense strands, and demonstrated how DNA modification data can be used in clustering of patient populations. Our findings revealed new epigenetic complexities but also highlighted the potential of DNA modification approaches in the search of heterogeneous causes of major psychiatric disease.

Original languageEnglish (US)
Pages (from-to)170-177
Number of pages8
JournalSchizophrenia bulletin
Volume42
Issue number1
DOIs
StatePublished - Jan 1 2016

Keywords

  • bipolar disorder
  • bisulfite sequencing
  • brain
  • epigenetics
  • padlock probes
  • schizophrenia

ASJC Scopus subject areas

  • Psychiatry and Mental health

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  • Cite this

    Pal, M., Ebrahimi, S., Oh, G., Khare, T., Zhang, A., Kaminsky, Z. A., Wang, S. C., & Petronis, A. (2016). High Precision DNA Modification Analysis of HCG9 in Major Psychosis. Schizophrenia bulletin, 42(1), 170-177. https://doi.org/10.1093/schbul/sbv079