High-performance liquid chromatographic procedures for the quantitative determination of paclitaxel (Taxol) in human urine

M. T. Huizing, H. Rosing, F. Koopman, A. C F Keung, H. M. Pinedo, J. H. Beijnen

Research output: Contribution to journalArticle

Abstract

A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the quantitative determination of paclitaxel in human urine. A comparison is made between solid-phase extraction (SPE) and liquid-liquid extraction (LLE) as sample pretreatment. The HPLC system consists of an APEX octyl analytical column and acetonitrile-methanol-0.2 μM ammonium acetate buffer pH 5 (4:1:5, v/v) as the mobile phase. Detection is performed by UV absorbance measurement at 227 nm. The SPE procedure involves extraction on Cyano Bond Elut columns. n-Butylchloride is the organic extraction fluid used for the LLE. The recoveries of paclitaxel in human urine are 79 and 75% for SPE and LLE, respectively. The accuracy for the LLE and SPE sample pretreatment procedures is 100.4 and 104.9%, respectively, at a 5 μg/ml drug concentration. The lower limit of quantitation is 0.01 μg/ml for SPE and 0.25 μg/ml for LLE. Stability data of paclitaxel in human urine are also presented.

Original languageEnglish (US)
Pages (from-to)373-382
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume664
Issue number2
DOIs
StatePublished - Feb 17 1995
Externally publishedYes

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Paclitaxel
Liquids
Methanol
Buffers
Recovery

ASJC Scopus subject areas

  • Chemistry(all)

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High-performance liquid chromatographic procedures for the quantitative determination of paclitaxel (Taxol) in human urine. / Huizing, M. T.; Rosing, H.; Koopman, F.; Keung, A. C F; Pinedo, H. M.; Beijnen, J. H.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 664, No. 2, 17.02.1995, p. 373-382.

Research output: Contribution to journalArticle

Huizing, M. T. ; Rosing, H. ; Koopman, F. ; Keung, A. C F ; Pinedo, H. M. ; Beijnen, J. H. / High-performance liquid chromatographic procedures for the quantitative determination of paclitaxel (Taxol) in human urine. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1995 ; Vol. 664, No. 2. pp. 373-382.
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