High-performance liquid chromatographic determination of the antitumor agent mitomycin c in human blood plasma

J. Den Hartigh; W. J. Van Oort; M. C Y M Bocken; H. M. Pinedo

doi:10.1016/S0003-2670(01)83960-X

High-performance liquid chromatographic determination of the antitumor agent mitomycin c in human blood plasma

J. Den Hartigh, W. J. Van Oort, M. C Y M Bocken, H. M. Pinedo

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Abstract

A high-performance liquid chromatographic method for the determination of the antitumor drug mitomycin C in blood plasma samples of cancer patients is described. The drug is extracted from the plasma with chloroform-2-propanol (1+1, w w) and chromatographed on a reversed-phase column with u.v. detection at 365 nm. The detection limit of the determination is 1 ng ml^-1 for 0.2-1.0 ml plasma samples. Preliminary results of a pharmacokinetic study show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical practice. The results obtained from multiwavelength detection suggest the existence of metabolites.

Original language	English (US)
Pages (from-to)	47-53
Number of pages	7
Journal	Analytica Chimica Acta
Volume	127
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2670(01)83960-X
State	Published - Jun 1 1981
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Analytical Chemistry
Spectroscopy
Environmental Chemistry

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title = "High-performance liquid chromatographic determination of the antitumor agent mitomycin c in human blood plasma",

abstract = "A high-performance liquid chromatographic method for the determination of the antitumor drug mitomycin C in blood plasma samples of cancer patients is described. The drug is extracted from the plasma with chloroform-2-propanol (1+1, w w) and chromatographed on a reversed-phase column with u.v. detection at 365 nm. The detection limit of the determination is 1 ng ml-1 for 0.2-1.0 ml plasma samples. Preliminary results of a pharmacokinetic study show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical practice. The results obtained from multiwavelength detection suggest the existence of metabolites.",

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AU - Den Hartigh, J.

AU - Van Oort, W. J.

AU - Bocken, M. C Y M

AU - Pinedo, H. M.

PY - 1981/6/1

Y1 - 1981/6/1

N2 - A high-performance liquid chromatographic method for the determination of the antitumor drug mitomycin C in blood plasma samples of cancer patients is described. The drug is extracted from the plasma with chloroform-2-propanol (1+1, w w) and chromatographed on a reversed-phase column with u.v. detection at 365 nm. The detection limit of the determination is 1 ng ml-1 for 0.2-1.0 ml plasma samples. Preliminary results of a pharmacokinetic study show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical practice. The results obtained from multiwavelength detection suggest the existence of metabolites.

AB - A high-performance liquid chromatographic method for the determination of the antitumor drug mitomycin C in blood plasma samples of cancer patients is described. The drug is extracted from the plasma with chloroform-2-propanol (1+1, w w) and chromatographed on a reversed-phase column with u.v. detection at 365 nm. The detection limit of the determination is 1 ng ml-1 for 0.2-1.0 ml plasma samples. Preliminary results of a pharmacokinetic study show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical practice. The results obtained from multiwavelength detection suggest the existence of metabolites.

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High-performance liquid chromatographic determination of the antitumor agent mitomycin c in human blood plasma

Abstract

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