High-performance liquid chromatographic determination of the antitumor agent mitomycin c in human blood plasma

J. Den Hartigh, W. J. Van Oort, M. C Y M Bocken, H. M. Pinedo

Research output: Contribution to journalArticle

Abstract

A high-performance liquid chromatographic method for the determination of the antitumor drug mitomycin C in blood plasma samples of cancer patients is described. The drug is extracted from the plasma with chloroform-2-propanol (1+1, w w) and chromatographed on a reversed-phase column with u.v. detection at 365 nm. The detection limit of the determination is 1 ng ml-1 for 0.2-1.0 ml plasma samples. Preliminary results of a pharmacokinetic study show that the sensitivity and selectivity of the assay are adequate for drug monitoring in clinical practice. The results obtained from multiwavelength detection suggest the existence of metabolites.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalAnalytica Chimica Acta
Volume127
Issue numberC
DOIs
StatePublished - Jun 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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