High-performance liquid chromatographic determination of liposomal nystatin in plasma and tissues for pharmacokinetic and tissue distribution studies

Andreas H. Groll, Diana Mickiene, Kathy Werner, Stephen C. Piscitelli, Thomas J. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

A reliable reversed-phase high-performance liquid chromatographic method was developed for the determination of liposomal nystatin in plasma. Nystatin is extracted by 1:2 (v/v) liquid-liquid extraction with methanol. Separation is achieved by HPLC after direct injection on a μBondapak(TM) C18 analytical column with a mobile phase composed of 10 mM sodium phosphate, 1 mM EDTA, 30% methanol and 30% acetonitrile adjusted to pH 6. Detection is by ultraviolet absorbance at 305 nm. Quantitation is based on the sum of the peak area concentration of the two major isomers of nystatin, which elute at 7.5-8.5 and 9.5-10.5 min. The assay was linear over the concentration range of 0.05 to 50 μg/ml. The lower limit of quantitation was 0.05 μg/ml, sufficient for investigating the plasma pharmacokinetics of liposomal nystatin in preclinical studies. Accuracies and intra- and inter-day precision showed good reproducibility. With minor modifications, this method also was used for assaying nystatin in various non-plasma body fluids and tissues. (C) 1999 Published by Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)51-62
Number of pages12
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume735
Issue number1
DOIs
StatePublished - Nov 26 1999

Keywords

  • Nystatin

ASJC Scopus subject areas

  • Chemistry(all)

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