High molecular weight neurofilament proteins are physiological substrates of adduction by the lipid peroxidation product hydroxynonenal

Takafumi Wataya, Akihiko Nunomura, Mark A. Smith, Sandra L. Siedlak, Peggy L R Harris, Shun Shimohama, Luke I. Szweda, Matthew A. Kaminski, Jesús Avila, Donald L. Price, Don W. Cleveland, Lawrence M. Sayre, George Perry

Research output: Contribution to journalArticle

Abstract

Protein adducts of the lipid peroxidation product trans-4-hydroxy-2-nonenal (HNE) are features of oxidative damage in neuronal cell bodies in Alzheimer's disease but are also seen in axons of normal as well as diseased individuals. In this study, focusing on the axons of the mouse sciatic nerve, we found that HNE adducts characterize axons of mice from birth to senility. Immunoblots of axonal proteins showed that HNE adducts are only detected in neurofilament heavy subunit (NFH) and, to a lesser extent, neurofilament medium subunit (NFM), both lysine-rich proteins, consistent with the adducts being limited to lysine residues. In vitro, HNE treatment of permeabilized sciatic nerve showed the same specificity, i.e. NFH and NFM are the only proteins that reacted with HNE, providing they are phosphorylated. Quantitative immunoblot analysis of two strains of mice ages 1-33 months showed that the levels of HNE adducts on NFH are consistent throughout life. Additionally, mice transgenic for human superoxide dismutase-1 with G85R mutation show no difference in HNE adduction to NFH compared with controls. Taken together, these studies indicate that HNE adduction to NFH is physiological, and its constancy from birth to senility as well as its dependence on phosphorylation argues that NFH and NFM modification may play a role in protecting the membrane-rich axon from toxic aldehydes resulting from oxidative damage.

Original languageEnglish (US)
Pages (from-to)4644-4648
Number of pages5
JournalJournal of Biological Chemistry
Volume277
Issue number7
DOIs
StatePublished - Feb 15 2002
Externally publishedYes

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Intermediate Filaments
Lipid Peroxidation
Lipids
Substrates
Axons
Sciatic Nerve
Lysine
Proteins
Parturition
Phosphorylation
neurofilament protein H
4-hydroxy-2-nonenal
Poisons
Protein Subunits
Aldehydes
Superoxide Dismutase
Transgenic Mice
Cells
Alzheimer Disease
Membranes

ASJC Scopus subject areas

  • Biochemistry

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High molecular weight neurofilament proteins are physiological substrates of adduction by the lipid peroxidation product hydroxynonenal. / Wataya, Takafumi; Nunomura, Akihiko; Smith, Mark A.; Siedlak, Sandra L.; Harris, Peggy L R; Shimohama, Shun; Szweda, Luke I.; Kaminski, Matthew A.; Avila, Jesús; Price, Donald L.; Cleveland, Don W.; Sayre, Lawrence M.; Perry, George.

In: Journal of Biological Chemistry, Vol. 277, No. 7, 15.02.2002, p. 4644-4648.

Research output: Contribution to journalArticle

Wataya, T, Nunomura, A, Smith, MA, Siedlak, SL, Harris, PLR, Shimohama, S, Szweda, LI, Kaminski, MA, Avila, J, Price, DL, Cleveland, DW, Sayre, LM & Perry, G 2002, 'High molecular weight neurofilament proteins are physiological substrates of adduction by the lipid peroxidation product hydroxynonenal', Journal of Biological Chemistry, vol. 277, no. 7, pp. 4644-4648. https://doi.org/10.1074/jbc.M110913200
Wataya, Takafumi ; Nunomura, Akihiko ; Smith, Mark A. ; Siedlak, Sandra L. ; Harris, Peggy L R ; Shimohama, Shun ; Szweda, Luke I. ; Kaminski, Matthew A. ; Avila, Jesús ; Price, Donald L. ; Cleveland, Don W. ; Sayre, Lawrence M. ; Perry, George. / High molecular weight neurofilament proteins are physiological substrates of adduction by the lipid peroxidation product hydroxynonenal. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 7. pp. 4644-4648.
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