High-frequency viscoelasticity of crosslinked actin filament networks measured by diffusing wave spectroscopy

Andre Palmer, Jingyuan Xu, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

We study the short-time relaxation dynamics of crosslinked and uncrosslinked networks of semi-flexible polymers using diffusing wave spectroscopy (DWS). The networks consist of concentrated solutions of actin filaments, crosslinked with increasing amounts of α-actinin. Actin filaments (F-actin) are long semi-flexible polymers with a contour length 1-100 μm and a persistence length of 5-15 μm; α-actinin is a small 200 kDa homodimer with two actin-binding sites. Using the large bandwidth of DWS, we measure the mean-square-displacement of 0.96 μm diameter microspheres imbedded in the polymer network, from which we extract the frequency-dependent viscoelastic moduli via a generalized Langevin equation. DWS measurements yield, in a single measurement, viscoelastic moduli at frequencies up to 105 Hz, almost three decades higher in frequency than probed by conventional mechanical rheology. Our measurements show that the magnitude of the small-frequency plateau modulus of F-actin is greatly enhanced in the presence of α-actinin, and that the frequency dependence of the viscoelastic moduli is much stronger at intermediate frequencies. However, the frequency-dependence of loss and storage moduli become similar for both crosslinked and uncrosslinked networks at large frequencies, G′(ω) ∝ G″(ω) ∝ ω0.75±0.08. This high-frequency behavior is due to the small-amplitude, large-frequency lateral fluctuations of actin filaments between entanglements.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalRheologica Acta
Volume37
Issue number2
StatePublished - Apr 1998

Fingerprint

viscoelasticity
Viscoelasticity
Actins
filaments
Spectroscopy
Actinin
Polymers
spectroscopy
Binding sites
Microspheres
Rheology
Relaxation time
Elastic moduli
Bandwidth
polymers
intermediate frequencies
Binding Sites
rheology
plateaus
relaxation time

Keywords

  • α-actinin
  • Actin
  • Diffusing wave spectroscopy
  • Semi flexible polymer

ASJC Scopus subject areas

  • Computational Mechanics
  • Mechanics of Materials

Cite this

High-frequency viscoelasticity of crosslinked actin filament networks measured by diffusing wave spectroscopy. / Palmer, Andre; Xu, Jingyuan; Wirtz, Denis.

In: Rheologica Acta, Vol. 37, No. 2, 04.1998, p. 97-106.

Research output: Contribution to journalArticle

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