High frequency retrotransposition in cultured mammalian cells

John V. Moran, Susan E. Holmes, Thierry P. Naas, Ralph J. DeBerardinis, Jef D. Boeke, Haig H. Kazazian

Research output: Contribution to journalArticle

Abstract

We previously isolated two human L1 elements (L1.2 and LRE2) as the progenitors of disease-producing insertions. Here, we show these elements can actively retrotranspose in cultured mammalian cells. When stably expressed from an episome in HeLa cells, both elements retrotransposed into a variety of chromosomal locations at a high frequency. The retrotransposed products resembled endogenous L1 insertions, since they were variably 5' truncated, ended in poly(A) tracts, and were flanked by target-site duplications or short deletions. Point mutations in conserved domains of the L1.2-encoded proteins reduced retrotransposition by 100- to 1000-fold. Remarkably, L1.2 also retrotransposed in a mouse cell line, suggesting a potential role for L1-based vectors in random insertional mutagenesis.

Original languageEnglish (US)
Pages (from-to)917-927
Number of pages11
JournalCell
Volume87
Issue number5
DOIs
StatePublished - Nov 29 1996

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Long Interspersed Nucleotide Elements
Poly A
Insertional Mutagenesis
HeLa Cells
Point Mutation
Cultured Cells
Plasmids
Cells
Cell Line
Mutagenesis
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Moran, J. V., Holmes, S. E., Naas, T. P., DeBerardinis, R. J., Boeke, J. D., & Kazazian, H. H. (1996). High frequency retrotransposition in cultured mammalian cells. Cell, 87(5), 917-927. https://doi.org/10.1016/S0092-8674(00)81998-4

High frequency retrotransposition in cultured mammalian cells. / Moran, John V.; Holmes, Susan E.; Naas, Thierry P.; DeBerardinis, Ralph J.; Boeke, Jef D.; Kazazian, Haig H.

In: Cell, Vol. 87, No. 5, 29.11.1996, p. 917-927.

Research output: Contribution to journalArticle

Moran, JV, Holmes, SE, Naas, TP, DeBerardinis, RJ, Boeke, JD & Kazazian, HH 1996, 'High frequency retrotransposition in cultured mammalian cells', Cell, vol. 87, no. 5, pp. 917-927. https://doi.org/10.1016/S0092-8674(00)81998-4
Moran JV, Holmes SE, Naas TP, DeBerardinis RJ, Boeke JD, Kazazian HH. High frequency retrotransposition in cultured mammalian cells. Cell. 1996 Nov 29;87(5):917-927. https://doi.org/10.1016/S0092-8674(00)81998-4
Moran, John V. ; Holmes, Susan E. ; Naas, Thierry P. ; DeBerardinis, Ralph J. ; Boeke, Jef D. ; Kazazian, Haig H. / High frequency retrotransposition in cultured mammalian cells. In: Cell. 1996 ; Vol. 87, No. 5. pp. 917-927.
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