High frequency of HLA-DQB1 *0301 allele in patients with pure ocular cicatricial pemphigoid

L. S. Chan, T. Wang, X. S. Wang, C. Hammerberg, K. D. Cooper

Research output: Contribution to journalArticle

Abstract

Two factors, its classification and its immunogenetics, may have contributed to an incomplete understanding of the pathogenic mechanism in the disease group commonly termed ‘cicatricial pemphigoid’. We have previously demonstrated that pure ocular cicatricial pemphigoid (OCP) is a unique clinical and immuno- pathological entity. In an effort to better define the immunogenetic characteristics for patients with pure OCP. we performed HLA typing for the class II MHC gene HLA-DQB 1*0301 allele, by amplifying genomic DNA extracted from patients with polymerase chain reaction, followed by hybridization with a sequence-specific oligonucleotide probe on dot-blotted and Southern-blotted amplified DNA samples. Ninety percent (9/10) of patients with pure OCP had the DQB1*0301 allele, in contrast to 52% (17/33) of normal individuals who had the DQB1*0301 allele. The high frequency of the HLA-DQB 1 *0301 allele in patients with pure OCP may point to a distinct immunogenetic pattern and possibly a distinct pathomechanism for the disease.

Original languageEnglish (US)
Pages (from-to)99-101
Number of pages3
JournalDermatology
Volume189
DOIs
StatePublished - Jan 1 1994

Keywords

  • Dot blot
  • HLA-DQB 1 * 0301 allele
  • Major histocompatibility complex
  • Polymerase chain reaction
  • Pure ocular cicatricial pemphigoid
  • Southern blot

ASJC Scopus subject areas

  • Dermatology

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    Chan, L. S., Wang, T., Wang, X. S., Hammerberg, C., & Cooper, K. D. (1994). High frequency of HLA-DQB1 *0301 allele in patients with pure ocular cicatricial pemphigoid. Dermatology, 189, 99-101. https://doi.org/10.1159/000246943