High-frequency deletion between homologous sequences during retrotransposition of Ty elements in Saccharomyces cerevisiae.

H. Xu, J. D. Boeke

Research output: Contribution to journalArticlepeer-review

Abstract

By following the fates of genetically marked Ty elements, we observed a very high frequency (80-90%) of deletion between directly repeated marker sequences during transposition. From blot hybridization analyses of Ty RNA and DNA species found in the Ty virus-like particles, we determined that the deletion events occurred during or immediately after reverse transcription of Ty RNA but before integration of Ty DNA. The results suggest that the Ty reverse-transcription machinery can recognize homologous sequences in the template. This capacity may be utilized in the replication and recombination processes of retrotransposons and retroviruses.

Original languageEnglish (US)
Pages (from-to)8553-8557
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number23
DOIs
StatePublished - Dec 1987

ASJC Scopus subject areas

  • General

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