High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan, Philip Hieter, Dorothy Sears, Amanda Burkhoff, Roger H. Reeves

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

Original languageEnglish (US)
Pages (from-to)125-127
Number of pages3
JournalGene
Volume106
Issue number1
DOIs
StatePublished - Sep 30 1991

Keywords

  • DNA-mediated transformation
  • YAC
  • deletion derivative
  • homologous recombination
  • restriction enzyme mapping

ASJC Scopus subject areas

  • Genetics

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