High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito; Mohamed Lehar; Zhao Bo Li; Natasha Braga; Alan D. King; Robin A. Samlan; Paul W. Flint

doi:10.1016/j.otohns.2006.04.003

High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito, Mohamed Lehar, Zhao Bo Li, Natasha Braga, Alan D. King, Robin A. Samlan, Paul W. Flint

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

Original language	English (US)
Pages (from-to)	209-214
Number of pages	6
Journal	Otolaryngology - Head and Neck Surgery
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/j.otohns.2006.04.003
State	Published - Aug 6 2006

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

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title = "High efficiency gene delivery into laryngeal muscle with bidirectional electroporation",

abstract = "Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.",

author = "Koichiro Saito and Mohamed Lehar and Li, {Zhao Bo} and Natasha Braga and King, {Alan D.} and Samlan, {Robin A.} and Flint, {Paul W.}",

note = "Funding Information: This work is funded by NIH/NIDCD grant DC04324-05. ",

year = "2006",

month = aug,

day = "6",

doi = "10.1016/j.otohns.2006.04.003",

language = "English (US)",

volume = "135",

pages = "209--214",

journal = "Otolaryngology - Head and Neck Surgery",

issn = "0194-5998",

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TY - JOUR

T1 - High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

AU - Saito, Koichiro

AU - Lehar, Mohamed

AU - Li, Zhao Bo

AU - Braga, Natasha

AU - King, Alan D.

AU - Samlan, Robin A.

AU - Flint, Paul W.

N1 - Funding Information: This work is funded by NIH/NIDCD grant DC04324-05.

PY - 2006/8/6

Y1 - 2006/8/6

N2 - Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

AB - Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

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High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

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