High efficiency capture of circulating tumor cells from patient blood using geometrically-enhanced differential immunocapture

Jason P. Gleghorn, Erica D. Pratt, Matthew S. Loftus, Benjamin E. Levy, Neil H. Bander, David M. Nanus, Paraskevi A. Giannakakou, Brian J. Kirby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we report the capture of circulating prostate tumor cells (PCTCs) from whole peripheral blood samples of patients with castrate-resistant prostate cancer. Using a microfluidic device geometry that maximizes streamline distortion and target cell-wall collisions, we have been able to capture cells with higher efficiencies and purities that exceed previously reported values. Potential applications for this device include capture of PCTCs to study the molecular mechanisms of clinical drug resistance, profiling of individual patients' CTCs in order to generate personalized chemotherapeutics, as well as real-time monitoring of chemotherapeutic efficacy using a non-invasive blood-based assay.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages657-659
Number of pages3
ISBN (Print)9780979806421
StatePublished - Jan 1 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

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Keywords

  • Blood
  • CTC
  • Immunocapture
  • Prostate

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Gleghorn, J. P., Pratt, E. D., Loftus, M. S., Levy, B. E., Bander, N. H., Nanus, D. M., Giannakakou, P. A., & Kirby, B. J. (2009). High efficiency capture of circulating tumor cells from patient blood using geometrically-enhanced differential immunocapture. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 657-659). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.