High dietary retinoic acid inhibits tumor promotion and malignant conversion in a two-stage skin carcinogenesis protocol using 7,12-dimethylbenz[a]anthracene as the initiator and mezerein as the tumor promoter in female SENCAR mice

Li Chuan Chen, Robert Tarone, Minh Huynh, Luigi M. De Luca

Research output: Contribution to journalArticle

Abstract

We studied the effect of dietary retinoic acid (RA) in a two-stage protocol of skin carcinogenesis in female SENCAR mice. At 3 weeks of age mice were initiated with 7,12-dimethylbenz[a]anthracene (DMBA, 20 μg) and promoted with either 12-O-tetradecanoylphorbol-13-acetate (TPA, 2μg) once per week or mezerein (MEZ, 4μg) twice per week for 20 weeks. At the week of DMBA initiation mice were also put on a purified diet containing either 3 (physiological dose) or 30 μg (pharmacological dose) of RA/g of diet. High dietary RA significantly inhibited papilloma yield but not incidence in the MEZ-promoted group. Papilloma incidence and yield were also lower in the MEZ- than in the TPA-treated groups. Cumulative carcinoma incidence and yield, and conversion efficiency (= (carcinomas/maximal papillomas) × 100%), were all decreased by high dietary RA in both MEZ- and TPA-treated groups. These results demonstrate that high dietary RA inhibited skin carcinogenesis in MEZ-promoted mice at the stages of tumor promotion and malignant conversion, while this inhibition occurred only at the malignant conversion stage in TPA-promoted mice.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalCancer Letters
Volume95
Issue number1-2
DOIs
StatePublished - Aug 16 1995
Externally publishedYes

Keywords

  • 12-O-Tetradecanoylphorbol-13-acetate
  • Malignant conversion
  • Mezerein
  • Retinoic acid
  • Skin carcinogenesis
  • Tumor promotion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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