High-density lipoprotein cholesterol efflux, nitration of apolipoprotein A-I, and endothelial function in obese women

Edward Vazquez, Amar A. Sethi, Lita Freeman, Gloria Zalos, Hira Chaudhry, Erin Haser, Brittany O. Aicher, Angel Aponte, Marjan Gucek, Gregory J. Kato, Myron A. Waclawiw, Alan T. Remaley, Richard O. Cannon

Research output: Contribution to journalArticle

Abstract

Subjects at risk of atherosclerosis might have dysfunctional high-density lipoprotein (HDL) despite normal cholesterol content in the plasma. We considered whether the efflux of excess cellular cholesterol to HDL from obese subjects is associated with impaired arterial endothelial function, a biomarker of cardiovascular risk. A total of 54 overweight (body mass index [BMI] 25 to 29.9 kg/m 2) or obese (BMI 2) women, aged 46 ± 11 years, were enrolled in a worksite wellness program. The HDL cholesterol averaged 57 ± 17 mg/dl and was inversely associated with the BMI (r = -0.419, p = 0.002). Endothelial function was assessed using brachial artery flow-mediated dilation. Cholesterol efflux from 3H- cholesterollabeled baby hamster kidney cells transfected with the adenosine triphosphate-binding cassette transporter 1 showed 8.2% to 22.5% cholesterol efflux within 18 hours when incubated with 1% serum and was positively correlated with brachial artery flow-mediated dilation (p 2 (r = 0.482, p = 0.004). This relation was independent of age, HDL or low-density lipoprotein cholesterol concentrations in plasma, blood pressure, or insulin resistance on stepwise multiple regression analysis (β = 0.31, R 2 = 0.21, p = 0.007). Nitration of apolipoprotein A-I tyrosine residues (using sandwich enzyme-linked immunosorbent assay) was significantly greater in women with a BMI 2 and the lowest cholesterol efflux than in women with a BMI of 25 to 29.9 kg/m 2 and the greatest cholesterol efflux (p = 0.01). In conclusion, we have shown that decreased cholesterol efflux by way of the adenosine triphosphate-binding cassette transporter 1 is associated with increased nitration of apolipoprotein A-I in HDL and is an independent predictor of impaired endothelial function in women with a BMI of 2. This finding suggests that the functional measures of HDL might be better markers for cardiovascular risk than the HDL cholesterol levels in this population.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalThe American Journal of Cardiology
Volume109
Issue number4
DOIs
StatePublished - Feb 15 2012
Externally publishedYes

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Apolipoprotein A-I
HDL Cholesterol
Body Mass Index
Cholesterol
HDL Lipoproteins
Brachial Artery
Dilatation
Adenosine Triphosphate
Health Promotion
Workplace
Cricetinae
LDL Cholesterol
Tyrosine
Insulin Resistance
Atherosclerosis
Biomarkers
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Blood Pressure
Kidney

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

High-density lipoprotein cholesterol efflux, nitration of apolipoprotein A-I, and endothelial function in obese women. / Vazquez, Edward; Sethi, Amar A.; Freeman, Lita; Zalos, Gloria; Chaudhry, Hira; Haser, Erin; Aicher, Brittany O.; Aponte, Angel; Gucek, Marjan; Kato, Gregory J.; Waclawiw, Myron A.; Remaley, Alan T.; Cannon, Richard O.

In: The American Journal of Cardiology, Vol. 109, No. 4, 15.02.2012, p. 527-532.

Research output: Contribution to journalArticle

Vazquez, E, Sethi, AA, Freeman, L, Zalos, G, Chaudhry, H, Haser, E, Aicher, BO, Aponte, A, Gucek, M, Kato, GJ, Waclawiw, MA, Remaley, AT & Cannon, RO 2012, 'High-density lipoprotein cholesterol efflux, nitration of apolipoprotein A-I, and endothelial function in obese women', The American Journal of Cardiology, vol. 109, no. 4, pp. 527-532. https://doi.org/10.1016/j.amjcard.2011.10.008
Vazquez, Edward ; Sethi, Amar A. ; Freeman, Lita ; Zalos, Gloria ; Chaudhry, Hira ; Haser, Erin ; Aicher, Brittany O. ; Aponte, Angel ; Gucek, Marjan ; Kato, Gregory J. ; Waclawiw, Myron A. ; Remaley, Alan T. ; Cannon, Richard O. / High-density lipoprotein cholesterol efflux, nitration of apolipoprotein A-I, and endothelial function in obese women. In: The American Journal of Cardiology. 2012 ; Vol. 109, No. 4. pp. 527-532.
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AU - Chaudhry, Hira

AU - Haser, Erin

AU - Aicher, Brittany O.

AU - Aponte, Angel

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AU - Remaley, Alan T.

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