TY - JOUR
T1 - High degree of Plasmodium vivax diversity in the peruvian amazon demonstrated by tandem repeat polymorphism analysis
AU - Kosek, Margaret
AU - Penataro Yori, Pablo
AU - Gilman, Robert H.
AU - Calderon, Maritza
AU - Zimic, Mirko
AU - Chuquiyauri, Raul
AU - Jeri, Cesar
AU - Pinedo-Cancino, Viviana
AU - Matthias, Michael A.
AU - Llanos-Cuentas, Alejandro
AU - Vinetz, Joseph M.
PY - 2012/4
Y1 - 2012/4
N2 - Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.
AB - Molecular tools to distinguish strains of Plasmodium vivax are important for studying the epidemiology of malaria transmission. Two sets of markers - tandem repeat (TR) polymorphisms and MSP3α - were used to study Plasmodium vivax in patients in the Peruvian Amazon region of Iquitos. Of 110 patients, 90 distinct haplotypes were distinguished using 9 TR markers. An MSP3α polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using HhaI and AluI revealed 8 and 9 profiles, respectively, and 36 profiles when analyzed in combination. Combining TR and PCR-RFLP markers, 101 distinct molecular profiles were distinguished among these 110 patients. Nine TR markers arrayed along a 100 kB stretch of a P. vivax chromosome containing the gene for circumsporozoite protein showed non-linear linkage disequilibrium (I SA = 0.03, P = 0.001). These findings demonstrate the potential use of TR markers for molecular epidemiology studies.
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U2 - 10.4269/ajtmh.2012.11-0627
DO - 10.4269/ajtmh.2012.11-0627
M3 - Article
C2 - 22492139
AN - SCOPUS:84860505028
SN - 0002-9637
VL - 86
SP - 580
EP - 586
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -