High‐ and Low‐Affinity Transport of D‐Glucose from Blood to Brain

Albert Gjedde

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Measurements of the unidirectional blood‐brain glucose flux in rat were incompatible with a single set of kinetic constants for transendothelial transport. At least two transfer mechanisms were present: a high‐affinity, low‐capacity system, and a low‐affinity, high‐capacity system. The low‐affinity system did not represent passive diffusion because it distinguished between D‐and L‐glucose. The Tmax and Km, for the high‐affinity system were 0.16 mmol 100 g−1 min−1 and 1 mM; for the low‐affinity system, ∼ 5 mmol 100 g−1 min−1 and ∼ 1 M. With these values, physiological glucose concentrations were not sufficient to saturate the low‐affinity system. In normoglycemia, therefore, three independent pathways of glucose transport from blood to brain appear to exist: a high‐affinity facilitated diffusion pathway of apparent permeability 235·10−7 cm s−1, a specific but nonsaturable diffusion pathway of permeability 85·10−7 cm s−l, and a nonspecifc passive diffusion pathway of permeability 2·10−7 cm s−1.

Original languageEnglish (US)
Pages (from-to)1463-1471
Number of pages9
JournalJournal of Neurochemistry
Volume36
Issue number4
DOIs
StatePublished - Apr 1981

Keywords

  • Blood‐brain glucose flux
  • Transfer mechanisms
  • Unidirectional brain uptake

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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