High affinity transport of choline into synaptosomes of rat brain

H. I. Yamamura, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

The accumulation of [3H] choline into synaptosome enriched homogenates of rat corpus striatum, cerebral cortex and cerebellum was studied at [3H] choline concentrations varying from 0.5 to 100 μM. The accumulation of [3H] choline in these brain regions was saturable. Kinetic analysis of the accumulation of the radiolabel was performed by double reciprocal plots and by least squares iterative fitting of a substrate velocity curve to the data. With both of these techniques, the data were best satisfied by two transport components, a high affinity uptake system with Km values of 1.4 μM (corpus striatum), and 3.1 μM (cerebral cortex) and a low affinity uptake system with respective Km values of 93 and 33 μM for these two brain regions. In the cerebellum, choline was accumulated only by the low affinity system. When striatal homogenates were fractionated further into synaptosomes and mitochondria and incubated with varying concentrations of [3H] choline, the high affinity component of choline uptake was localized to the synaptosomal fraction. The high affinity uptake system required sodium was sensitive to various metabolic inhibitors, and was associated with considerable formation of [3H]acetylcholine. The low affinity uptake system was much less dependent on sodium, and was not associated with a marked degree of [3H]acetylcholine formation. Hemicholinium 3 and acetylcholine were potent inhibitors of the high affinity uptake system. A variety of evidence suggests that the high affinity transport represents a selective accumulation of choline by cholinergic neurons, while the low affinity uptake system has a less specific function.

Original languageEnglish (US)
Pages (from-to)1355-1374
Number of pages20
JournalJournal of Neurochemistry
Volume21
Issue number6
StatePublished - 1973
Externally publishedYes

Fingerprint

Synaptosomes
Choline
Rats
Brain
Corpus Striatum
Acetylcholine
Cerebral Cortex
Cerebellum
Sodium
Hemicholinium 3
Mitochondria
Cholinergic Neurons
Least-Squares Analysis
Cholinergic Agents
Neurons
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

High affinity transport of choline into synaptosomes of rat brain. / Yamamura, H. I.; Snyder, Solomon H.

In: Journal of Neurochemistry, Vol. 21, No. 6, 1973, p. 1355-1374.

Research output: Contribution to journalArticle

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