High-affinity IgE receptor-β chain expression in human mast cells

Akira Matsuda, Yoshimichi Okayama, Nobuyuki Ebihara, Norihiko Yokoi, Peisong Gao, Junji Hamuro, Julian M. Hopkin, Shigeru Kinoshita

Research output: Contribution to journalArticle

Abstract

The high-affinity IgE receptor (FcεRI)-β gene is one of the atopy-associated genes, but its biological significance is largely unknown. In this study, we generated the anti-FcεRI-β chain antibody to clarify β-chain protein expression in human mast cells. The FcεRI-β antibody showed specific binding to a 27 kDa protein with Western blotting and membrane bound immunostaining using cultured mast cells. Monomeric IgE sensitization increased β-chain expression as well as mature α-chain expression in mast cells. Upregulation of β-chain expression with monomeric IgE treatment suggests possible roles of FcεRI-β protein as an atopy-related molecule.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalJournal of Immunological Methods
Volume336
Issue number2
DOIs
Publication statusPublished - Jul 31 2008

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Keywords

  • Atopic diseases
  • IgE receptor
  • Immunoblotting
  • Immunocytochemistry
  • Mast cells

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Matsuda, A., Okayama, Y., Ebihara, N., Yokoi, N., Gao, P., Hamuro, J., ... Kinoshita, S. (2008). High-affinity IgE receptor-β chain expression in human mast cells. Journal of Immunological Methods, 336(2), 229-234. https://doi.org/10.1016/j.jim.2008.05.006