High affinity as a disease determinant factor in anti-GM1 antibodies: Comparative characterization of experimentally induced vs. disease-associated antibodies

P. H.H. Lopez, A. M. Villa, R. E.P. Sica, G. A. Nores

Research output: Contribution to journalArticlepeer-review

Abstract

Elevated titers of serum anti-GM1 antibodies of IgG isotype are found frequently in patients with Guillain-Barré syndrome. Much evidence indicates that these autoantibodies are involved in disease progression, but their exact function and the mechanism of their appearance are still unclear. In an attempt to reproduce "ganglioside syndrome", the experimental model of neuropathy developed by Nagai et al. (Neurosci. Lett. 2 (1976) 107), rabbits were intensively immunized with GM1 in complete Freund adjuvant (CFA). High titers of anti-GM1 antibodies were produced, with class switch and affinity maturation indicating an elaborate immune response. Unexpectedly, the rabbits did not show any clinical symptoms of neuropathy. Relatively affinities of both IgM and IgG antibodies were significantly lower than those of similar antibodies from neuropathy patients. These results suggest the existence of a threshold value above which affinity of anti-GM1 antibodies becomes an important factor in disease induction. The absence of neuropathy symptoms in rabbits may be explained by absence of these high-affinity anti-GM1 antibodies.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalJournal of Neuroimmunology
Volume128
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Anti-GM antibodies
  • GM
  • Gangliosides
  • Guillain-Barré syndrome
  • Neuropathy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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