High aerobic glycolysis of rat hepatoma cells in culture

Role of mitochondrial hexokinase

E. Bustamante, Peter L Pedersen

Research output: Contribution to journalArticle

Abstract

A tumorigenic anchorage-dependent cell line (H-91) was established in culture from an azo-dye-induced rat ascites hepatoma. When grown in a glucose-containing medium the cells exhibit high rates of lactic acid production characteristic of rapidly growing tumor cells. However, when glucose is replaced with galactose the cells grow equally well but exhibit only moderately elevated rates of lactic acid production. The molecular basis for this observation cannot be attributed to differences in permeability because initial rates of glucose and galactose entry into hepatoma cells are identical. Rather, the activity of hexokinase (ATP:D hexose 6-phosphotransferase, EC 2.7.1.1) is found to be high in hepatoma cells, about 20-fold higher than that of control and regenerating rat liver. Moreover, tumor hexokinase activity is not inhibited by low concentrations (

Original languageEnglish (US)
Pages (from-to)3735-3739
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number9
StatePublished - 1977

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Hexokinase
Glycolysis
Hepatocellular Carcinoma
Cell Culture Techniques
Galactose
Glucose
Lactic Acid
Azo Compounds
Ascites
Permeability
Neoplasms
Cell Line
Liver

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

High aerobic glycolysis of rat hepatoma cells in culture : Role of mitochondrial hexokinase. / Bustamante, E.; Pedersen, Peter L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 9, 1977, p. 3735-3739.

Research output: Contribution to journalArticle

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