HIF 1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor

Thomas Fillies, Richard Werkmeister, Paul J. van Diest, Burkhard Brandt, Ulrich Joos, Horst Buerger

Research output: Contribution to journalArticle

Abstract

Background: Hypoxia-inducible factor 1 (HIF-1) is a transcription factor, which plays a central role in biologic processes under hypoxic conditions, especially concerning tumour angiogenesis. HIF-1α is the relevant, oxygen-dependent subunit and its overexpression has been associated with a poor prognosis in a variety of malignant tumours. Therefore, HIF-1α expression in early stage oral carcinomas was evaluated in relation to established clinico-pathological features in order to determine its value as a prognostic marker. Methods: 85 patients with histologically proven surgically treated T1/2 squamous cell carcinoma (SCC) of the oral floor were eligible for the study. Tumor specimens were investigated by means of tissue micro arrays (TMAs) and immunohistochemistry for the expression of HIF-1. Correlations between clinical features and the expression of HIF-1 were evaluated by Kaplan-Meier curves, log-rank tests and multivariate Cox regression analysis. Results: HIF-1α was frequently overexpressed in a probably non-hypoxia related fashion. The expression of HIF-1α was related with a significantly improved 5-year survival rate (p <0.01) and a significantly increased disease free period (p = 0.01) independent from nodal status and tumour size. In primary node negative T1/T2 SCC of the oral floor, absence of HIF-1α expression specified a subgroup of high-risk patients (p <0.05). Conclusion: HIF-1α overexpression is an indicator of favourable prognosis in T1 and T2 SCC of the oral floor. Node negative patients lacking HIF-1α expression may therefore be considered for adjuvant radiotherapy.

Original languageEnglish (US)
Article number84
JournalBMC Cancer
Volume5
DOIs
StatePublished - Jul 21 2005
Externally publishedYes

Fingerprint

Mouth Floor
Hypoxia-Inducible Factor 1
Squamous Cell Carcinoma
Neoplasms
Oral Stage
Adjuvant Radiotherapy
Transcription Factors
Survival Rate
Immunohistochemistry
Regression Analysis
Oxygen
Carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Genetics

Cite this

HIF 1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor. / Fillies, Thomas; Werkmeister, Richard; van Diest, Paul J.; Brandt, Burkhard; Joos, Ulrich; Buerger, Horst.

In: BMC Cancer, Vol. 5, 84, 21.07.2005.

Research output: Contribution to journalArticle

Fillies, Thomas ; Werkmeister, Richard ; van Diest, Paul J. ; Brandt, Burkhard ; Joos, Ulrich ; Buerger, Horst. / HIF 1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor. In: BMC Cancer. 2005 ; Vol. 5.
@article{31e4be00a4e74b34a2bb5e51d2e6339f,
title = "HIF 1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor",
abstract = "Background: Hypoxia-inducible factor 1 (HIF-1) is a transcription factor, which plays a central role in biologic processes under hypoxic conditions, especially concerning tumour angiogenesis. HIF-1α is the relevant, oxygen-dependent subunit and its overexpression has been associated with a poor prognosis in a variety of malignant tumours. Therefore, HIF-1α expression in early stage oral carcinomas was evaluated in relation to established clinico-pathological features in order to determine its value as a prognostic marker. Methods: 85 patients with histologically proven surgically treated T1/2 squamous cell carcinoma (SCC) of the oral floor were eligible for the study. Tumor specimens were investigated by means of tissue micro arrays (TMAs) and immunohistochemistry for the expression of HIF-1. Correlations between clinical features and the expression of HIF-1 were evaluated by Kaplan-Meier curves, log-rank tests and multivariate Cox regression analysis. Results: HIF-1α was frequently overexpressed in a probably non-hypoxia related fashion. The expression of HIF-1α was related with a significantly improved 5-year survival rate (p <0.01) and a significantly increased disease free period (p = 0.01) independent from nodal status and tumour size. In primary node negative T1/T2 SCC of the oral floor, absence of HIF-1α expression specified a subgroup of high-risk patients (p <0.05). Conclusion: HIF-1α overexpression is an indicator of favourable prognosis in T1 and T2 SCC of the oral floor. Node negative patients lacking HIF-1α expression may therefore be considered for adjuvant radiotherapy.",
author = "Thomas Fillies and Richard Werkmeister and {van Diest}, {Paul J.} and Burkhard Brandt and Ulrich Joos and Horst Buerger",
year = "2005",
month = "7",
day = "21",
doi = "10.1186/1471-2407-5-84",
language = "English (US)",
volume = "5",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central",

}

TY - JOUR

T1 - HIF 1-alpha overexpression indicates a good prognosis in early stage squamous cell carcinomas of the oral floor

AU - Fillies, Thomas

AU - Werkmeister, Richard

AU - van Diest, Paul J.

AU - Brandt, Burkhard

AU - Joos, Ulrich

AU - Buerger, Horst

PY - 2005/7/21

Y1 - 2005/7/21

N2 - Background: Hypoxia-inducible factor 1 (HIF-1) is a transcription factor, which plays a central role in biologic processes under hypoxic conditions, especially concerning tumour angiogenesis. HIF-1α is the relevant, oxygen-dependent subunit and its overexpression has been associated with a poor prognosis in a variety of malignant tumours. Therefore, HIF-1α expression in early stage oral carcinomas was evaluated in relation to established clinico-pathological features in order to determine its value as a prognostic marker. Methods: 85 patients with histologically proven surgically treated T1/2 squamous cell carcinoma (SCC) of the oral floor were eligible for the study. Tumor specimens were investigated by means of tissue micro arrays (TMAs) and immunohistochemistry for the expression of HIF-1. Correlations between clinical features and the expression of HIF-1 were evaluated by Kaplan-Meier curves, log-rank tests and multivariate Cox regression analysis. Results: HIF-1α was frequently overexpressed in a probably non-hypoxia related fashion. The expression of HIF-1α was related with a significantly improved 5-year survival rate (p <0.01) and a significantly increased disease free period (p = 0.01) independent from nodal status and tumour size. In primary node negative T1/T2 SCC of the oral floor, absence of HIF-1α expression specified a subgroup of high-risk patients (p <0.05). Conclusion: HIF-1α overexpression is an indicator of favourable prognosis in T1 and T2 SCC of the oral floor. Node negative patients lacking HIF-1α expression may therefore be considered for adjuvant radiotherapy.

AB - Background: Hypoxia-inducible factor 1 (HIF-1) is a transcription factor, which plays a central role in biologic processes under hypoxic conditions, especially concerning tumour angiogenesis. HIF-1α is the relevant, oxygen-dependent subunit and its overexpression has been associated with a poor prognosis in a variety of malignant tumours. Therefore, HIF-1α expression in early stage oral carcinomas was evaluated in relation to established clinico-pathological features in order to determine its value as a prognostic marker. Methods: 85 patients with histologically proven surgically treated T1/2 squamous cell carcinoma (SCC) of the oral floor were eligible for the study. Tumor specimens were investigated by means of tissue micro arrays (TMAs) and immunohistochemistry for the expression of HIF-1. Correlations between clinical features and the expression of HIF-1 were evaluated by Kaplan-Meier curves, log-rank tests and multivariate Cox regression analysis. Results: HIF-1α was frequently overexpressed in a probably non-hypoxia related fashion. The expression of HIF-1α was related with a significantly improved 5-year survival rate (p <0.01) and a significantly increased disease free period (p = 0.01) independent from nodal status and tumour size. In primary node negative T1/T2 SCC of the oral floor, absence of HIF-1α expression specified a subgroup of high-risk patients (p <0.05). Conclusion: HIF-1α overexpression is an indicator of favourable prognosis in T1 and T2 SCC of the oral floor. Node negative patients lacking HIF-1α expression may therefore be considered for adjuvant radiotherapy.

UR - http://www.scopus.com/inward/record.url?scp=26844502441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26844502441&partnerID=8YFLogxK

U2 - 10.1186/1471-2407-5-84

DO - 10.1186/1471-2407-5-84

M3 - Article

C2 - 16035955

AN - SCOPUS:26844502441

VL - 5

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

M1 - 84

ER -