HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors

Hilde L K Janssen, Karin M G Haustermans, Debbie Sprong, Gerard Blommestijn, Ingrid Hofland, Frank J. Hoebers, Elke Blijweert, James A. Raleigh, Gregg L Semenza, Mahesh A. Varia, Alfons J. Balm, Marie Louise F Van Velthuysen, Pierre Delaere, Raf Sciot, Adrian C. Begg

Research output: Contribution to journalArticle

Abstract

Purpose: Tumor hypoxia measured by microelectrodes has been shown to indicate poor patient outcome. Here we investigated four potentially more widely applicable immunohistochemical parameters of tumor oxygenation and perfusion in human head-and-neck tumors. Methods: Twenty patients with squamous cell carcinomas of the head and neck treated with primary surgery were injected with pimonidazole and IdUrd the evening before operation. Consecutive paraffin-embedded sections were stained for blood vessels, pimonidazole, IdUrd, and HIF-1α. IdUrd labeling and Ki-67 labeling around individual blood vessels were scored. The spatial relationship between HIF-1α and pimonidazole was studied, as well as the distribution of both markers as a function of distance from the nearest blood vessel. Results: Measurement of all four parameters (diffusion-limited fraction, pimonidazole fraction, HIF-1α fraction, IdUrd-negative vessels) was feasible, and a significant difference between tumors was found for all parameters. IdUrd-labeled cells were absent around some vessels, indicating lack of perfusion, because these regions were positive for Ki-67. There was a positive correlation between diffusion-limited fraction and pimonidazole area for all images from all tumors, although no correlation for mean values per tumor. Colocalization of pimonidazole and HIF-1α was low (0.02%-25%). Most expression profiles showed a more homogenous distribution for HIF-1α than pimonidazole. There was no significant correlation between the pimonidazole and HIF-1α fractions in the 10 tumors studied. Conclusions: Simultaneous immunohistochemical measurements related to hypoxia and perfusion are feasible (and easily applicable) in resected human tumors. The different geographic distributions of HIF-1α and pimonidazole indicate that HIF-1α might not be suitable as a marker for chronic hypoxia. Each parameter will be correlated with outcome in a larger ongoing study on head-and-neck tumors treated with surgery with or without postoperative radiotherapy.

Original languageEnglish (US)
Pages (from-to)1537-1549
Number of pages13
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume54
Issue number5
DOIs
StatePublished - Dec 1 2002

Fingerprint

Idoxuridine
hypoxia
Neck
tumors
Perfusion
Head
estimates
Neoplasms
blood vessels
Blood Vessels
surgery
markers
marking
vessels
geographic distribution
pimonidazole
Hypoxia
evening
oxygenation
Microelectrodes

Keywords

  • HIF-1α
  • Hypoxia
  • Pimonidazole
  • Predictive assay
  • Radiation therapy

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Janssen, H. L. K., Haustermans, K. M. G., Sprong, D., Blommestijn, G., Hofland, I., Hoebers, F. J., ... Begg, A. C. (2002). HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors. International Journal of Radiation Oncology, Biology, Physics, 54(5), 1537-1549. https://doi.org/10.1016/S0360-3016(02)03935-4

HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors. / Janssen, Hilde L K; Haustermans, Karin M G; Sprong, Debbie; Blommestijn, Gerard; Hofland, Ingrid; Hoebers, Frank J.; Blijweert, Elke; Raleigh, James A.; Semenza, Gregg L; Varia, Mahesh A.; Balm, Alfons J.; Van Velthuysen, Marie Louise F; Delaere, Pierre; Sciot, Raf; Begg, Adrian C.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 54, No. 5, 01.12.2002, p. 1537-1549.

Research output: Contribution to journalArticle

Janssen, HLK, Haustermans, KMG, Sprong, D, Blommestijn, G, Hofland, I, Hoebers, FJ, Blijweert, E, Raleigh, JA, Semenza, GL, Varia, MA, Balm, AJ, Van Velthuysen, MLF, Delaere, P, Sciot, R & Begg, AC 2002, 'HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors', International Journal of Radiation Oncology, Biology, Physics, vol. 54, no. 5, pp. 1537-1549. https://doi.org/10.1016/S0360-3016(02)03935-4
Janssen, Hilde L K ; Haustermans, Karin M G ; Sprong, Debbie ; Blommestijn, Gerard ; Hofland, Ingrid ; Hoebers, Frank J. ; Blijweert, Elke ; Raleigh, James A. ; Semenza, Gregg L ; Varia, Mahesh A. ; Balm, Alfons J. ; Van Velthuysen, Marie Louise F ; Delaere, Pierre ; Sciot, Raf ; Begg, Adrian C. / HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors. In: International Journal of Radiation Oncology, Biology, Physics. 2002 ; Vol. 54, No. 5. pp. 1537-1549.
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T1 - HIF-1α, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors

AU - Janssen, Hilde L K

AU - Haustermans, Karin M G

AU - Sprong, Debbie

AU - Blommestijn, Gerard

AU - Hofland, Ingrid

AU - Hoebers, Frank J.

AU - Blijweert, Elke

AU - Raleigh, James A.

AU - Semenza, Gregg L

AU - Varia, Mahesh A.

AU - Balm, Alfons J.

AU - Van Velthuysen, Marie Louise F

AU - Delaere, Pierre

AU - Sciot, Raf

AU - Begg, Adrian C.

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N2 - Purpose: Tumor hypoxia measured by microelectrodes has been shown to indicate poor patient outcome. Here we investigated four potentially more widely applicable immunohistochemical parameters of tumor oxygenation and perfusion in human head-and-neck tumors. Methods: Twenty patients with squamous cell carcinomas of the head and neck treated with primary surgery were injected with pimonidazole and IdUrd the evening before operation. Consecutive paraffin-embedded sections were stained for blood vessels, pimonidazole, IdUrd, and HIF-1α. IdUrd labeling and Ki-67 labeling around individual blood vessels were scored. The spatial relationship between HIF-1α and pimonidazole was studied, as well as the distribution of both markers as a function of distance from the nearest blood vessel. Results: Measurement of all four parameters (diffusion-limited fraction, pimonidazole fraction, HIF-1α fraction, IdUrd-negative vessels) was feasible, and a significant difference between tumors was found for all parameters. IdUrd-labeled cells were absent around some vessels, indicating lack of perfusion, because these regions were positive for Ki-67. There was a positive correlation between diffusion-limited fraction and pimonidazole area for all images from all tumors, although no correlation for mean values per tumor. Colocalization of pimonidazole and HIF-1α was low (0.02%-25%). Most expression profiles showed a more homogenous distribution for HIF-1α than pimonidazole. There was no significant correlation between the pimonidazole and HIF-1α fractions in the 10 tumors studied. Conclusions: Simultaneous immunohistochemical measurements related to hypoxia and perfusion are feasible (and easily applicable) in resected human tumors. The different geographic distributions of HIF-1α and pimonidazole indicate that HIF-1α might not be suitable as a marker for chronic hypoxia. Each parameter will be correlated with outcome in a larger ongoing study on head-and-neck tumors treated with surgery with or without postoperative radiotherapy.

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KW - HIF-1α

KW - Hypoxia

KW - Pimonidazole

KW - Predictive assay

KW - Radiation therapy

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