Hiding in plain view: Genetic profiling reveals decades old cross contamination of bladder cancer cell line ku7 with hela

Wolfgang Jäger, Yutaka Horiguchi, Jay Shah, Tetsutaro Hayashi, Shannon Awrey, Kilian M. Gust, Boris A. Hadaschik, Yoshiyuki Matsui, Shawn Anderson, Robert H. Bell, Susan Ettinger, Alan I. So, Martin E. Gleave, I. Ling Lee, Colin P. Dinney, Masaaki Tachibana, David McConkey, Peter C. Black

Research output: Contribution to journalArticle

Abstract

Purpose KU7 is a popular urothelial carcinoma cell line that was isolated from the bladder of a patient at Keio University in 1980. It has subsequently been widely used in laboratories around the world. We describe how routine cell line authentication revealed that KU7 was cross contaminated almost 30 years ago with HeLa, a cervical carcinoma cell line. Materials and Methods Presumed KU7 clones dating from 1984 to 1999 were provided by M.D. Anderson Cancer Center, Vancouver Prostate Centre, Kyoto University, Tokyo Medical University and Keio University. HeLa was obtained from ATCC®. Genomic DNA was isolated and short tandem repeat analysis was performed at the M.D. Anderson Cancer Center Characterized Cell Line Core Facility, Johns Hopkins University Fragment Analysis Facility and RIKEN BioResource Center, Ibaraki, Japan. Comparative genomic hybridization was performed on a platform (Agilent Technologies, Santa Clara, California) at Vancouver Prostate Centre. Results The short tandem repeat profile of all KU7 clones was an exact match with that of HeLa. Comparative genomic hybridization of all samples revealed an abundance of shared chromosomal aberrations. Slight differences in some genomic areas were explained by genomic drift in different KU7 clones separated by many years. Conclusions Our analysis identified that cross contamination of KU7 with HeLa occurred before 1984 at the source institution. All KU7 clones in the urological literature should be considered HeLa and experimental results should be viewed in this light. Our results emphasize the need to authenticate cell lines in oncological research.

Original languageEnglish (US)
Pages (from-to)1404-1409
Number of pages6
JournalJournal of Urology
Volume190
Issue number4
DOIs
Publication statusPublished - Oct 2013
Externally publishedYes

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Keywords

  • carcinoma
  • DNA contamination
  • HeLa cells
  • urinary bladder
  • urothelium

ASJC Scopus subject areas

  • Urology

Cite this

Jäger, W., Horiguchi, Y., Shah, J., Hayashi, T., Awrey, S., Gust, K. M., ... Black, P. C. (2013). Hiding in plain view: Genetic profiling reveals decades old cross contamination of bladder cancer cell line ku7 with hela. Journal of Urology, 190(4), 1404-1409. https://doi.org/10.1016/j.juro.2013.03.009