Hfq proximity and orientation controls RNA annealing

Subrata Panja, Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

Regulation of bacterial gene networks by small non-coding RNAs (sRNAs) requires base pairing with messenger RNA (mRNA) targets, which is facilitated by Hfq protein. Hfq is recruited to sRNAs and mRNAs through U-rich-and A-rich-binding sites, respectively, but their distance from the sRNA-mRNA complementary region varies widely among different genes. To determine whether distance and binding orientation affect Hfq's chaperone function, we engineered 'toy' RNAs containing strong Hfq-binding sites at defined distances from the complementary target site. We show that RNA annealing is fastest when the distal face of Hfq binds an A-rich sequence immediately 3′ of the target. This recruitment advantage is lost when Hfq binds >20nt away from the target, but is partially restored by secondary structure that shortens this distance. Although recruitment through Hfq's distal face accelerates RNA annealing, tight binding of six Us to Hfq's proximal face inhibits annealing. Finally, we show that ectopic A-rich motifs dramatically accelerate base pairing between DsrA sRNA and a minimal rpoS mRNA in the presence of Hfq, demonstrating that proximity and orientation predict the activity of Hfq on long RNAs.

Original languageEnglish (US)
Pages (from-to)8690-8697
Number of pages8
JournalNucleic Acids Research
Volume40
Issue number17
DOIs
StatePublished - Sep 2012

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Small Untranslated RNA
RNA
Messenger RNA
Base Pairing
Binding Sites
Bacterial Genes
Play and Playthings
Gene Regulatory Networks
Genes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Hfq proximity and orientation controls RNA annealing. / Panja, Subrata; Woodson, Sarah A.

In: Nucleic Acids Research, Vol. 40, No. 17, 09.2012, p. 8690-8697.

Research output: Contribution to journalArticle

Panja, Subrata ; Woodson, Sarah A. / Hfq proximity and orientation controls RNA annealing. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 17. pp. 8690-8697.
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