Hexose transport in microvascular endothelial cells cultured from bovine retina

A. Lorris Betz, Phillip D. Bowman, Gary William Goldstein

Research output: Contribution to journalArticle

Abstract

The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-d-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional glucose transport inhibitors such as phloretin, phlorizin and cytochalasin B but not by agents that deplete intracellular ATP (2,4-dinitrophenol) or that abolish the sodium gradient (ouabain). Uptake of 3MG by the cells could be stimulated by preloading with 50 mm-glucose, a phenomenon known as counter-transport. Insulin had no effect on 3MG uptake in the cells even after prolonged insulin deprivation. These results demonstrate the existence of a facilitative-diffusion type of glucose transport system in endothelial cells of the retinal microvasculature. Studies with 2-deoxy-d-glucose demonstrated that transport was not rate limiting for metabolism and that the endothelial cells contain free sugar when exposed to an extracellular sugar concentration in the physiologic range. The presence of this free sugar within the cell is necessary for efficient transendothelial transfer of glucose from blood to retina but it could also provide the basis for capillary damage in diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
JournalExperimental Eye Research
Volume36
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

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Hexoses
3-O-Methylglucose
Retina
Endothelial Cells
Glucose
Blood-Retinal Barrier
Phloretin
Insulin
Phlorhizin
2,4-Dinitrophenol
Cytochalasin B
Ouabain
Microvessels
Blood Glucose
Diabetes Mellitus
Adenosine Triphosphate
Sodium

Keywords

  • 2-deoxyglucose
  • 3-O-methylglucose
  • blood-retinal barrier
  • cytochalasin B
  • endothelial cells
  • glucose
  • insulin
  • metabolism
  • phloretin
  • phlorizin
  • transport

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Hexose transport in microvascular endothelial cells cultured from bovine retina. / Betz, A. Lorris; Bowman, Phillip D.; Goldstein, Gary William.

In: Experimental Eye Research, Vol. 36, No. 2, 1983, p. 269-277.

Research output: Contribution to journalArticle

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