Hev b 5 and Hev b 13 as allergen markers to estimate the allergenic potency of latex gloves

Hoong Yeet Yeang, Siti Arija M. Arif, Monika Raulf-Heimsoth, Yin Ho Loke, Ingrid Sander, Siti Hawa Sulong, Chee Heng Lau, Robert G. Hamilton

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Background Sensitization to natural rubber latex has been linked to proteins from medical latex gloves. Various assays to estimate the amount of residual allergenic proteins extractable from latex gloves to assess their potential exposure hazard have inherent weaknesses. Objective This investigation was aimed at developing 2-site immunoenzymetric assays and identifying appropriate protein markers to assess the allergenic potential of latex gloves. Methods The presence of 6 latex allergens - Hev b 1, 2, 3, 5, 6, and 13 - was measured in a cross-section of commercial latex medical gloves by using monoclonal and polyclonal antibody-based 2-site immunoenzymetric assays. The overall allergenic potential of these gloves was assessed by IgE-inhibition assay. Stepwise multiple regression analyses were performed to identify marker allergens that best explained the variation in latex glove allergenicity. Results All 6 latex allergens were detected in at least some of the glove samples. Hev b 5 and Hev b 13 were identified as the marker allergens that combined best to explain the variation in the glove allergenicity. The significant multiple correlation (R=0.855) between these 2 markers and glove allergenic potency forms the basis of an assay to gauge latex glove allergenicity. Conclusion The overall allergenic potential of latex gloves can be estimated by using Hev b 5 and Hev b 13 as indicator allergens. The correlation between glove allergenicity and the level of these allergens was maintained for low-protein gloves (<200 μg/g). This estimation of glove allergenicity was superior to that obtained by using total protein readings.

Original languageEnglish (US)
Pages (from-to)593-598
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Issue number3
StatePublished - Sep 2004
Externally publishedYes


  • BGFA
  • Hev b 13
  • Hev b 5
  • IEMA
  • IUIS
  • Immunoenzymetric assay
  • International Union of Immunological Societies
  • JHU
  • Latex allergy
  • Research Institute for Occupational Medicine
  • allergen
  • immunoassay
  • latex glove
  • protein
  • step-wise multiple regression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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