Heterogeneous localization of protein kinase C in rat brain

Autoradiographic analysis of phorbol ester receptor binding

Research output: Contribution to journalArticle

Abstract

Protein kinase C is a calcium- and phospholipid-stimulated enzyme present in high concentration in the brain. Phorbol esters are potent tumor promoters that bind to specific receptors with high affinity. Several lines of evidence indicate that the phorbol ester receptor is identical to protein kinase C. To determine the distribution of protein kinase C, we have localized phorbol ester receptors in the rat brain by autoradiography, using [3H]phorbol 12,13-dibutyrate ([3H]PDBu) and have performed a variety of lesions to assess the nature of the cellular elements possessing the binding sites. The [3H]PDBu binding sites in the rat brain are discretely localized and primarily associated with neurons. Evidence is presented for localization to intrinsic neurons of the cortex and hippocampus, terminals of the striatonigral projection, a projection to the molecular layer of the dentate gyrus, and to dendrites of Purkinje cells.

Original languageEnglish (US)
Pages (from-to)199-207
Number of pages9
JournalJournal of Neuroscience
Volume6
Issue number1
StatePublished - 1986

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Protein Kinase C
Brain
Binding Sites
Phorbol 12,13-Dibutyrate
Neurons
Purkinje Cells
Dentate Gyrus
Phorbol Esters
Dendrites
Autoradiography
Carcinogens
Hippocampus
Phospholipids
Calcium
Enzymes
phorbol ester receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Heterogeneous localization of protein kinase C in rat brain: Autoradiographic analysis of phorbol ester receptor binding",
abstract = "Protein kinase C is a calcium- and phospholipid-stimulated enzyme present in high concentration in the brain. Phorbol esters are potent tumor promoters that bind to specific receptors with high affinity. Several lines of evidence indicate that the phorbol ester receptor is identical to protein kinase C. To determine the distribution of protein kinase C, we have localized phorbol ester receptors in the rat brain by autoradiography, using [3H]phorbol 12,13-dibutyrate ([3H]PDBu) and have performed a variety of lesions to assess the nature of the cellular elements possessing the binding sites. The [3H]PDBu binding sites in the rat brain are discretely localized and primarily associated with neurons. Evidence is presented for localization to intrinsic neurons of the cortex and hippocampus, terminals of the striatonigral projection, a projection to the molecular layer of the dentate gyrus, and to dendrites of Purkinje cells.",
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T2 - Autoradiographic analysis of phorbol ester receptor binding

AU - Worley, Paul F

AU - Baraban, Jay M

AU - Snyder, Solomon H

PY - 1986

Y1 - 1986

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AB - Protein kinase C is a calcium- and phospholipid-stimulated enzyme present in high concentration in the brain. Phorbol esters are potent tumor promoters that bind to specific receptors with high affinity. Several lines of evidence indicate that the phorbol ester receptor is identical to protein kinase C. To determine the distribution of protein kinase C, we have localized phorbol ester receptors in the rat brain by autoradiography, using [3H]phorbol 12,13-dibutyrate ([3H]PDBu) and have performed a variety of lesions to assess the nature of the cellular elements possessing the binding sites. The [3H]PDBu binding sites in the rat brain are discretely localized and primarily associated with neurons. Evidence is presented for localization to intrinsic neurons of the cortex and hippocampus, terminals of the striatonigral projection, a projection to the molecular layer of the dentate gyrus, and to dendrites of Purkinje cells.

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