Heterogeneity of metallo and serine extracellular proteinases in oral clinical isolates of Candida albicans in HIV-positive and healthy children from Rio de Janeiro, Brazil

Edja Maria Melo De Brito Costa, André Luis Souza Dos Santos, Abel Silveira Cardoso, Maristela Barbosa Portela, Celina Monteiro Abreu, Celuta Sales Alviano, Allen Norton Hagler, Rosangela Maria De Araújo Soares

Research output: Contribution to journalArticlepeer-review

Abstract

Candida yeasts frequently cause life-threatening systemic infections in immunocompromised hosts. In the present study, gelatin-SDS-PAGE analysis was used to characterize extracellular proteinases in 44 oral clinical isolates of Candida albicans from HIV-positive (29/50) and healthy children (15/50). Our survey indicates that these oral clinical isolates of C. albicans have complex extracellular proteolytic activity profiles, which illustrates the heterogeneity of this species. We showed four distinct proteolytic patterns composed of distinct serine (30-58 kDa) and metalloproteinase (64-95 kDa) activities, based on the inhibition profile with phenylmethylsulfonyl fluoride and 1,10-phenanthroline, respectively. This is the first report on secreted serine and metalloproteinases present in the culture supernatant fluids of C. albicans; however, we did not observe a significant correlation between proteolytic profile expressed by the C. albicans isolates from HIV-positive children and CD4+ T cell count and plasma viral load.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume38
Issue number2
DOIs
StatePublished - Sep 22 2003
Externally publishedYes

Keywords

  • CD4 T cell
  • Candida albicans
  • Extracellular proteolytic activity
  • HIV-infected child
  • Oral clinical isolate
  • Serine and metalloproteinase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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