Heterogeneity of anti-phospholipid and anti-endothelial cell antibodies

Hua Shan, June Goldman, Gina Cunto, Joseph Manuppello, Irwin Chaiken, Douglas B. Cines, Leslie E. Silberstein

Research output: Contribution to journalArticle

Abstract

The role of the anti-phospholipid antibodies (APLA) and anti- endothelial cell antibodies (AECA) in the pathogenesis of anti-phospholipid syndrome (APS) is unclear. Differences in the reported involvement of APLA may be due, in part, to the polyclonal nature of these antibodies and the use of serum and serum fractions for analysis. To circumvent this issue, we generated monoclonal antibodies (MAB) from three patients with APS and two healthy controls. We then compared the antigen binding patterns and the heavy chain variable region (V(H)) DNA sequences of the MAB derived from patients with APS to those from healthy controls. The results of this study indicate that APLA and AECA comprise a highly heterogeneous population of antibodies with respect to the antigens they recognize, as well as V(H) gene usage. MAB derived from patients with APS do not differ from those derived from normal individuals based on either antigen recognition or V(H) gene usage. These results suggest the importance of additional predisposing factors in the pathogenesis of APS.

Original languageEnglish (US)
Pages (from-to)651-660
Number of pages10
JournalJournal of Autoimmunity
Volume11
Issue number6
DOIs
Publication statusPublished - Dec 1998
Externally publishedYes

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Keywords

  • Anti- phospholipid syndrome
  • Anti-endothelial cell antibodies
  • Anti-phospholipid antibodies
  • Autoimmunity
  • Ig variable gene usage

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Shan, H., Goldman, J., Cunto, G., Manuppello, J., Chaiken, I., Cines, D. B., & Silberstein, L. E. (1998). Heterogeneity of anti-phospholipid and anti-endothelial cell antibodies. Journal of Autoimmunity, 11(6), 651-660. https://doi.org/10.1006/jaut.1998.0242