Heterogeneity in Macular Corneal Dystrophy

Deepak P. Edward, Beatrice Y J T Yue, Joel Sugar, Eugene M A Thonar, Nirmala Sunderraj, E. Stock Lee, Mark O M Tso

Research output: Contribution to journalArticle

Abstract

Macular corneal dystrophy is an autosomal recessive disorder in which abnormal deposits in the corneal stroma have been identified. We examined the corneal buttons of 12 patients, who had clinical features of macular dystrophy, by histochemical staining, transmission electron microscopy, and immunohistochemical techniques. All corneas exhibited positive staining with Muller Mowry’s colloidal iron. Using monoclonal antibodies 1/20/ 5-D-4, J-10, J-19, and J-36 that recognize specific sites on the sulfated keratan sulfate molecule, we stained corneal sections by an avidin-biotin-peroxidase complex method and identified two groups of macular corneal dystrophy. One group consisting of four corneas reacted positively with all four antibodies, and the other group consisting of eight corneas did not react with any of the antibodies used. These results confirmed those recently presented by Yang et al that there may be subgroups of macular dystrophy that can be identified by immunohistochemical methods. Also, serum levels of sulfated keratan sulfate were determined in seven patients. One patient who displayed a normal level of serum keratan sulfate had positive corneal immunoreactivity. Of the six patients who lacked serum keratan sulfate, four showed negative and two had positive corneal immunostaining, suggesting at least three sub-groups in the disease. An attempt was made to correlate the clinical features, histochemical-staining characteristics, and ultrastructural morphology with the immunoreactivity to keratan sulfate antibodies, but no correlations could be made.

Original languageEnglish (US)
Pages (from-to)1579-1583
Number of pages5
JournalArchives of Ophthalmology
Volume106
Issue number11
DOIs
StatePublished - 1988
Externally publishedYes

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Keratan Sulfate
Cornea
Macular Degeneration
Staining and Labeling
Antibodies
Serum
Corneal Stroma
Avidin
Biotin
Transmission Electron Microscopy
Peroxidase
Iron
Monoclonal Antibodies
Corneal type 1 Macular dystrophy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Edward, D. P., Yue, B. Y. J. T., Sugar, J., Thonar, E. M. A., Sunderraj, N., Stock Lee, E., & Tso, M. O. M. (1988). Heterogeneity in Macular Corneal Dystrophy. Archives of Ophthalmology, 106(11), 1579-1583. https://doi.org/10.1001/archopht.1988.01060140747049

Heterogeneity in Macular Corneal Dystrophy. / Edward, Deepak P.; Yue, Beatrice Y J T; Sugar, Joel; Thonar, Eugene M A; Sunderraj, Nirmala; Stock Lee, E.; Tso, Mark O M.

In: Archives of Ophthalmology, Vol. 106, No. 11, 1988, p. 1579-1583.

Research output: Contribution to journalArticle

Edward, DP, Yue, BYJT, Sugar, J, Thonar, EMA, Sunderraj, N, Stock Lee, E & Tso, MOM 1988, 'Heterogeneity in Macular Corneal Dystrophy', Archives of Ophthalmology, vol. 106, no. 11, pp. 1579-1583. https://doi.org/10.1001/archopht.1988.01060140747049
Edward DP, Yue BYJT, Sugar J, Thonar EMA, Sunderraj N, Stock Lee E et al. Heterogeneity in Macular Corneal Dystrophy. Archives of Ophthalmology. 1988;106(11):1579-1583. https://doi.org/10.1001/archopht.1988.01060140747049
Edward, Deepak P. ; Yue, Beatrice Y J T ; Sugar, Joel ; Thonar, Eugene M A ; Sunderraj, Nirmala ; Stock Lee, E. ; Tso, Mark O M. / Heterogeneity in Macular Corneal Dystrophy. In: Archives of Ophthalmology. 1988 ; Vol. 106, No. 11. pp. 1579-1583.
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